Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/ElaA-DUF1778
Location 29825..30582 Replicon plasmid unnamed
Accession NZ_CP026794
Organism Shigella flexneri strain 74-1170

Toxin (Protein)


Gene name tacT Uniprot ID D2AJG5
Locus tag C1P81_RS24615 Protein ID WP_000501974.1
Coordinates 29825..30310 (-) Length 162 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID Q31SN8
Locus tag C1P81_RS24620 Protein ID WP_011114751.1
Coordinates 30298..30582 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C1P81_RS24585 25349..25773 - 425 Protein_29 hypothetical protein -
C1P81_RS24590 26001..26639 - 639 WP_000502854.1 ParB N-terminal domain-containing protein -
C1P81_RS24595 26624..26767 - 144 Protein_31 DUF3440 domain-containing protein -
C1P81_RS24610 29371..29652 + 282 Protein_34 IS4/IS5 family transposase -
C1P81_RS24615 29825..30310 - 486 WP_000501974.1 GNAT family N-acetyltransferase Toxin
C1P81_RS24620 30298..30582 - 285 WP_011114751.1 DUF1778 domain-containing protein Antitoxin
C1P81_RS26515 31484..31648 + 165 WP_001346193.1 hypothetical protein -
C1P81_RS24645 31902..33503 - 1602 WP_005063916.1 IS66-like element ISSfl3 family transposase -
C1P81_RS24650 33500..33850 - 351 WP_005048975.1 IS66 family insertion sequence element accessory protein TnpB -
C1P81_RS24655 33847..34521 - 675 WP_001088287.1 IS66-like element accessory protein TnpA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - ipaH7.8 / ipaH7.8 / ipaH4.5 / ipaH4.5 / ipaH4.5 / ipaH1.4 / ospD3/senA / ospC1 / ospC3 / ipaJ / ipaA / ipaD / ipaC / ipaB / ipgC / ipgB1 / ipgA / icsB / ipgD / ipgE / ipgF / mxiG / mxiH / mxiI / mxiJ / mxiK / mxiN / mxiL / mxiM / mxiE / mxiD / mxiC / mxiA / spa15 / spa47 / spa13 / spa32 / spa33 / spa24 / spa9 / spa29 / spa40 / virA / icsA/virG / papC / ospI / ipaH9.8 / ospG / ipaH1.4 / ospE1 / nleE / icsP/sopA / ospB / ospC4 / ospC2 / ospD2 / ospF / ospD1 / ipgB2 / ospE2 / ipaH2.5 / ipaH7.8 / ospC2 / ipaH7.8 / ipaH4.5 / ospD3/senA / ospD3/senA / ospC1 / ospC1 1..251323 251323
- inside IScluster/Tn - ipaH7.8 / ipaH7.8 / ipaH4.5 / ipaH4.5 / ipaH4.5 / ipaH1.4 / ospD3/senA / ospC1 / ospC3 468..43266 42798


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(27-151)

Antitoxin

Vibrio phage ICP1, Orf50

(12-88)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17814.63 Da        Isoelectric Point: 9.8822

>T95990 WP_000501974.1 NZ_CP026794:c30310-29825 [Shigella flexneri]
MGCVTAPEPLSSFHQVAEFVSSEAVLDDWLKQKELKNQAIGATRTFVVCRKGTQQIVGFYSLATGSVNHTEATGNLRRNM
PDPIPVIILARLAVDVSFRGKGLGADLLHDAVRRCYRVAENIGVRAIMVHALTENAKQFYIHHGFKPSKTQVQTLFLKLP
Q

Download         Length: 486 bp

>T95990 NZ_CP026794:c30310-29825 [Shigella flexneri]
ATGGGATGTGTAACTGCACCAGAACCTTTATCCTCATTTCATCAGGTCGCTGAATTTGTCAGCAGTGAAGCTGTACTTGA
TGACTGGTTAAAGCAGAAGGAGCTAAAAAATCAGGCAATCGGAGCCACCCGGACTTTTGTTGTATGCAGAAAAGGTACGC
AACAAATAGTCGGTTTTTACTCACTGGCCACTGGTAGTGTCAATCATACAGAAGCTACAGGTAACCTTCGGCGCAATATG
CCAGACCCCATTCCTGTAATTATACTTGCACGCCTGGCTGTAGATGTGTCATTTCGAGGAAAAGGGCTTGGCGCGGATTT
ACTTCATGATGCAGTTCGTCGTTGTTACAGGGTTGCCGAAAATATCGGTGTGCGTGCAATTATGGTTCATGCGCTTACGG
AAAACGCAAAACAGTTTTATATTCATCATGGTTTTAAGCCATCAAAAACTCAGGTGCAAACATTGTTTCTTAAACTCCCT
CAATAG

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10871.52 Da        Isoelectric Point: 4.6812

>AT95990 WP_011114751.1 NZ_CP026794:c30582-30298 [Shigella flexneri]
MIYGGFMKSGVQLNLRARESQRILIDAAAEILHKSRTDFILEMACKAAEDVILDRRVFNFNDRQYEEFIEMLDAPVADDP
AIEKLLARKPQWDV

Download         Length: 285 bp

>AT95990 NZ_CP026794:c30582-30298 [Shigella flexneri]
ATGATATACGGAGGCTTTATGAAATCAGGTGTTCAGCTCAATCTCAGAGCAAGAGAATCACAGCGGATACTCATTGATGC
TGCTGCAGAAATTCTTCATAAGTCGCGTACAGATTTCATTCTGGAAATGGCCTGTAAAGCTGCTGAAGATGTTATTCTCG
ACCGGCGGGTGTTTAATTTTAATGACAGGCAATATGAAGAGTTCATTGAAATGCTTGATGCTCCGGTTGCAGATGATCCT
GCCATCGAAAAATTGCTGGCAAGGAAACCTCAATGGGATGTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A822PN28

Antitoxin

Source ID Structure
AlphaFold DB A0A4V1CUJ1

References