Detailed information of TA system TA095955

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 32907..33432
• Replicon: plasmid unnamed
• Accession: NZ_CP026791
• Organism: Shigella flexneri strain 61-4982

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: Q326Z8
• Locus tag: C1P80_RS00240
• Protein ID: WP_001159860.1
• Coordinates: 33127..33432 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: Q7BEK0
• Locus tag: C1P80_RS00235
• Protein ID: WP_000813626.1
• Coordinates: 32907..33125 (+)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C1P80_RS00190	28634..28818	+	185	Protein_32	ATP-binding protein	-
C1P80_RS00195	28918..29127	-	210	Protein_33	peptidoglycan-binding protein	-
C1P80_RS00200	29177..29356	-	180	Protein_34	MFS transporter	-
C1P80_RS00210	29880..30164	+	285	WP_024260696.1	ribbon-helix-helix domain-containing protein	-
C1P80_RS00215	30164..30439	+	276	WP_000421262.1	type II toxin-antitoxin system RelE/ParE family toxin	-
C1P80_RS00225	30665..31362	-	698	Protein_37	IS1 family transposase	-
C1P80_RS00230	31418..32371	+	954	Protein_38	IS66 family transposase	-
C1P80_RS00235	32907..33125	+	219	WP_000813626.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
C1P80_RS00240	33127..33432	+	306	WP_001159860.1	type II toxin-antitoxin system toxin CcdB	Toxin
C1P80_RS00245	33468..33563	+	96	Protein_41	transposase domain-containing protein	-
C1P80_RS00250	33825..35462	+	1638	WP_000936806.1	T3SS effector E3 ubiquitin-protein ligase IpaH9.8	-
C1P80_RS00255	35670..35939	+	270	Protein_43	hypothetical protein	-
C1P80_RS24600	35936..36162	+	227	Protein_44	type II toxin-antitoxin system RelE/ParE family toxin	-
C1P80_RS00265	36191..36781	+	591	WP_000705601.1	type III secretion system effector protein kinase OspG	-
C1P80_RS24350	37120..37404	+	285	Protein_46	IS66 family insertion sequence element accessory protein TnpB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	espC / ospC2 / ipaH2.5 / ospI / ipaH9.8 / ospG	1..59834	59834
	-	inside	IScluster/Tn	-	espC / ospC2 / ipaH2.5 / ospI	4240..32371	28131

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11736.59 Da
• Isoelectric Point: 6.4674

>T95955 WP_001159860.1 NZ_CP026791:33127-33432 [Shigella flexneri]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPIFVIGEEV
ADLSHRENDIKNAINLMFWGI

Nucleotide

• Length: 306 bp

>T95955 NZ_CP026791:33127-33432 [Shigella flexneri]
ATGCAGTTTAAGGTTTACGCCTATAAAAGAGAGAGTCGTTATCGTCTGTTTGTGGATGTACAGAGTGACATTATTGACAC
GCCCGGACGACGGATGGTGATCCCCCTGGCCAGTGCCCGCCTGCTGTCAGATAAAGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACTGATATGGCCAGTGTGCCGATCTTCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8333.36 Da
• Isoelectric Point: 6.2838

>AT95955 WP_000813626.1 NZ_CP026791:32907-33125 [Shigella flexneri]
MKQRITVTIDSDSYQLLKSANVNISGLVNTAMQKEARRLRAERWQAENQQGMAEIARFIEMNGSFADENRDW

Nucleotide

• Length: 219 bp

>AT95955 NZ_CP026791:32907-33125 [Shigella flexneri]
ATGAAGCAGCGTATTACTGTCACTATAGACAGTGACAGCTATCAGTTACTTAAATCCGCAAACGTGAATATCTCAGGCCT
GGTAAATACGGCCATGCAAAAAGAGGCCCGCCGTCTGCGTGCGGAACGCTGGCAGGCTGAAAATCAGCAGGGGATGGCTG
AGATCGCCCGTTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA

Structures

Toxin

• AlphaFold DB: A0A4P7TTN3

Antitoxin

• AlphaFold DB: Q7BEK0