Detailed information of TA system TA095954

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 29880..30439
• Replicon: plasmid unnamed
• Accession: NZ_CP026791
• Organism: Shigella flexneri strain 61-4982

Toxin (Protein)

• Gene name: relE
• Uniprot ID: D2AJT2
• Locus tag: C1P80_RS00215
• Protein ID: WP_000421262.1
• Coordinates: 30164..30439 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: C1P80_RS00210
• Protein ID: WP_024260696.1
• Coordinates: 29880..30164 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C1P80_RS00170	25361..25710	+	350	Protein_28	IS66 family insertion sequence element accessory protein TnpB	-
C1P80_RS00175	25707..26258	+	552	Protein_29	transposase	-
C1P80_RS00180	26316..27472	+	1157	WP_134796986.1	IS3 family transposase	-
C1P80_RS00185	27516..28445	+	930	Protein_31	IS66-like element ISSfl3 family transposase	-
C1P80_RS00190	28634..28818	+	185	Protein_32	ATP-binding protein	-
C1P80_RS00195	28918..29127	-	210	Protein_33	peptidoglycan-binding protein	-
C1P80_RS00200	29177..29356	-	180	Protein_34	MFS transporter	-
C1P80_RS00210	29880..30164	+	285	WP_024260696.1	ribbon-helix-helix domain-containing protein	Antitoxin
C1P80_RS00215	30164..30439	+	276	WP_000421262.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C1P80_RS00225	30665..31362	-	698	Protein_37	IS1 family transposase	-
C1P80_RS00230	31418..32371	+	954	Protein_38	IS66 family transposase	-
C1P80_RS00235	32907..33125	+	219	WP_000813626.1	type II toxin-antitoxin system antitoxin CcdA	-
C1P80_RS00240	33127..33432	+	306	WP_001159860.1	type II toxin-antitoxin system toxin CcdB	-
C1P80_RS00245	33468..33563	+	96	Protein_41	transposase domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	espC / ospC2 / ipaH2.5 / ospI / ipaH9.8 / ospG	1..59834	59834
	-	inside	IScluster/Tn	-	espC / ospC2 / ipaH2.5 / ospI	4240..32371	28131

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (14-51)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10766.48 Da
• Isoelectric Point: 9.9324

>T95954 WP_000421262.1 NZ_CP026791:30164-30439 [Shigella flexneri]
MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFTGEYEIRYELTGQTIY
VLRLWHTRENR

Nucleotide

• Length: 276 bp

>T95954 NZ_CP026791:30164-30439 [Shigella flexneri]
ATGGAACTGAAGTGGACCAGTAAGGCACTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTGGCCAGTAAACCTGC
TGCCGCCAGAACGGTGCAGTCCCTGACACAGGCGCCGGTCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTACTGGCGAGTACGAAATCCGTTACGAACTTACTGGCCAGACTATTTAT
GTATTGCGCCTGTGGCACACACGAGAGAACAGGTAG

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10513.93 Da
• Isoelectric Point: 6.2150

>AT95954 WP_024260696.1 NZ_CP026791:29880-30164 [Shigella flexneri]
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQSW
ADSLSTDHPLPVPR

Nucleotide

• Length: 285 bp

>AT95954 NZ_CP026791:29880-30164 [Shigella flexneri]
ATGCAAATGAAAAACAATACCGCACAAGCAACAAAAGTAATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGCTCCCGGGGCTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAAGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATCGACCATCAGGCTGTACAGTCCTGG
GCGGACAGCCTCAGTACTGACCATCCGTTACCGGTGCCACGCTGA

Structures

Toxin

• AlphaFold DB: A0A822PVM3