Detailed information of TA system TA095953

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/ElaA-DUF1778
• Location: 16944..17719
• Replicon: plasmid unnamed
• Accession: NZ_CP026791
• Organism: Shigella flexneri strain 61-4982

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: Q7BEG9
• Locus tag: C1P80_RS00120
• Protein ID: WP_000405245.1
• Coordinates: 17237..17719 (+)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: Q7BEG8
• Locus tag: C1P80_RS00115
• Protein ID: WP_010921625.1
• Coordinates: 16944..17246 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C1P80_RS00080	12262..12905	-	644	Protein_13	IS1 family transposase	-
C1P80_RS00085	13309..14763	+	1455	WP_075332747.1	type 3 secretion system effector OspC2	-
C1P80_RS24210	14912..15040	-	129	Protein_15	IS5/IS1182 family transposase	-
C1P80_RS00105	16400..16672	-	273	WP_000019158.1	hypothetical protein	-
C1P80_RS00115	16944..17246	+	303	WP_010921625.1	DUF1778 domain-containing protein	Antitoxin
C1P80_RS00120	17237..17719	+	483	WP_000405245.1	GNAT family N-acetyltransferase	Toxin
C1P80_RS00125	17787..18310	+	524	Protein_20	IS3-like element ISSfl11 family transposase	-
C1P80_RS00130	18353..19562	-	1210	Protein_21	IS3 family transposase	-
C1P80_RS00135	19614..21305	-	1692	WP_134796984.1	IpaH family type III secretion system E3 ubiquitin--protein ligase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	espC / ospC2 / ipaH2.5 / ospI / ipaH9.8 / ospG	1..59834	59834
	-	inside	IScluster/Tn	-	espC / ospC2 / ipaH2.5 / ospI	4240..32371	28131

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (29-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (19-94)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17750.52 Da
• Isoelectric Point: 7.0382

>T95953 WP_000405245.1 NZ_CP026791:17237-17719 [Shigella flexneri]
MEINVTAPALLTDEHILQPFDCGNEVLSNWLRGRAMKNQMLNASRTFVICLEDTLRIVGYYSLATGSVTHAELGRSLRHN
MPNPVPVVLLGRLAVDVCTQGHGFGKWLLSDAIHRVVNLADQVGIKAVMVHAIDDDARAFYERFGFVQSVVAPNTLFYKV

Nucleotide

• Length: 483 bp

>T95953 NZ_CP026791:17237-17719 [Shigella flexneri]
ATGGAAATAAATGTCACCGCGCCAGCATTGTTGACGGATGAGCATATACTTCAGCCATTTGACTGTGGAAATGAGGTGCT
AAGTAACTGGTTACGTGGTCGGGCTATGAAAAACCAGATGCTCAATGCTTCTCGCACGTTCGTTATTTGCTTGGAGGATA
CTTTACGTATTGTGGGATACTACTCATTAGCTACTGGTTCCGTCACTCATGCCGAGCTTGGTCGCAGTTTGCGGCATAAC
ATGCCCAATCCTGTCCCTGTTGTTCTGTTAGGGCGTCTGGCTGTTGATGTTTGTACCCAGGGGCATGGTTTCGGTAAATG
GCTACTAAGTGATGCCATTCACCGGGTTGTCAATCTGGCTGATCAGGTTGGCATTAAGGCTGTTATGGTACATGCAATTG
ATGATGACGCCAGAGCATTTTATGAGCGTTTTGGTTTTGTTCAGTCAGTTGTGGCACCTAATACCTTGTTTTATAAAGTC
TGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11689.28 Da
• Isoelectric Point: 4.9167

>AT95953 WP_010921625.1 NZ_CP026791:16944-17246 [Shigella flexneri]
MEVFMSTAASVRKTPREHQINIRATDEERAVIDYAASLVNKNRTDFIMELAYQEAKNIILDQRLFVLDNERYDSFITQLE
APVQNAEGRERLMAVKPEWK

Nucleotide

• Length: 303 bp

>AT95953 NZ_CP026791:16944-17246 [Shigella flexneri]
ATGGAGGTTTTTATGTCTACAGCTGCAAGCGTCCGTAAAACACCGCGAGAACATCAGATTAACATCCGGGCAACTGATGA
GGAACGAGCCGTTATTGATTATGCTGCGAGCTTGGTCAATAAGAACCGTACCGATTTCATCATGGAACTGGCGTACCAGG
AAGCTAAAAACATTATCCTGGATCAACGTTTATTTGTGCTGGATAACGAACGTTATGATTCGTTTATTACGCAACTGGAA
GCTCCTGTGCAGAATGCAGAAGGGCGTGAACGTCTGATGGCGGTAAAGCCTGAATGGAAATAA

Structures

Toxin

• AlphaFold DB: Q7BEG9

Antitoxin

• AlphaFold DB: Q7BEG8