Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 2756352..2756983 Replicon chromosome
Accession NZ_CP026788
Organism Shigella flexneri 2a strain ATCC 29903

Toxin (Protein)


Gene name hicA Uniprot ID A0A822PMR8
Locus tag C1P79_RS14545 Protein ID WP_001259384.1
Coordinates 2756708..2756983 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A822PD91
Locus tag C1P79_RS14540 Protein ID WP_000593553.1
Coordinates 2756352..2756711 (-) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C1P79_RS14515 (2752484) 2752484..2753428 + 945 WP_000947076.1 nickel ABC transporter permease subunit NikB -
C1P79_RS14520 (2753425) 2753425..2754258 + 834 WP_001008973.1 nickel ABC transporter permease subunit NikC -
C1P79_RS14525 (2754258) 2754258..2755022 + 765 WP_001136253.1 nickel import ATP-binding protein NikD -
C1P79_RS14530 (2755019) 2755019..2755825 + 807 WP_000173660.1 nickel import ATP-binding protein NikE -
C1P79_RS14535 (2755831) 2755831..2756232 + 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
C1P79_RS14540 (2756352) 2756352..2756711 - 360 WP_000593553.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
C1P79_RS14545 (2756708) 2756708..2756983 - 276 WP_001259384.1 type II toxin-antitoxin system HicA family toxin Toxin
C1P79_RS14550 (2757042) 2757042..2758166 - 1125 WP_001216257.1 ABC transporter permease -
C1P79_RS14555 (2758166) 2758166..2760901 - 2736 WP_000149173.1 ribosome-associated ATPase/putative transporter RbbA -
C1P79_RS14560 (2760898) 2760898..2761965 - 1068 WP_000361470.1 HlyD family secretion protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-81)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10146.89 Da        Isoelectric Point: 10.3748

>T95947 WP_001259384.1 NZ_CP026788:c2756983-2756708 [Shigella flexneri 2a]
MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
CDWLLSIGVKP

Download         Length: 276 bp

>T95947 NZ_CP026788:c2756983-2756708 [Shigella flexneri 2a]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGCAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
TGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13474.34 Da        Isoelectric Point: 4.9169

>AT95947 WP_000593553.1 NZ_CP026788:c2756711-2756352 [Shigella flexneri 2a]
MIKLKTPNSMEIAEQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT95947 NZ_CP026788:c2756711-2756352 [Shigella flexneri 2a]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGAGCAACCAGCTGTTATTACCTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGTATTCAGGGGCTGCAAAAAGAAGGAG
AGCTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A822PMR8

Antitoxin

Source ID Structure
AlphaFold DB A0A822PD91

References