Detailed information of TA system TA095930

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 109469..110244
• Replicon: plasmid unnamed
• Accession: NZ_CP026787
• Organism: Shigella dysenteriae strain NCTC 9718

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: Q326V1
• Locus tag: C1P65_RS24610
• Protein ID: WP_000405248.1
• Coordinates: 109469..109951 (-)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: -
• Locus tag: C1P65_RS24615
• Protein ID: WP_162147618.1
• Coordinates: 109942..110244 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C1P65_RS24590	105836..107044	-	1209	WP_119178644.1	IS91 family transposase	-
C1P65_RS24595	107025..107297	-	273	WP_000019161.1	hypothetical protein	-
C1P65_RS26595	107372..108169	+	798	Protein_131	T3SS effector E3 ubiquitin-protein ligase IpaH1.4	-
C1P65_RS26600	108152..108835	+	684	Protein_132	IS3 family transposase	-
C1P65_RS24605	108878..109401	-	524	Protein_133	transposase	-
C1P65_RS24610	109469..109951	-	483	WP_000405248.1	GNAT family N-acetyltransferase	Toxin
C1P65_RS24615	109942..110244	-	303	WP_162147618.1	DUF1778 domain-containing protein	Antitoxin
C1P65_RS24620	110658..111392	-	735	Protein_136	IS21-like element IS100kyp family helper ATPase IstB	-
C1P65_RS24625	111525..112667	-	1143	Protein_137	IS91 family transposase	-
C1P65_RS24630	112648..112920	-	273	WP_000019161.1	hypothetical protein	-
C1P65_RS24640	113003..113527	-	525	Protein_139	transposase	-
C1P65_RS24645	113664..113837	-	174	WP_011379008.1	transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	nleE / icsP/sopA / ospB / ospC2 / ospD2 / ospF / ospD1 / ipgB2 / ipaH4.5 / ipaH7.8 / ospI / ipaH9.8 / ospG / espC / ipaH2.5 / ipaH2.5 / ospE2 / espC / ospC3 / ipaJ / ipaA / ipaD / ipaC / ipaB / ipgC / ipgB1 / ipgA / icsB / ipgD / ipgE / ipgF / mxiG / mxiH / mxiI / mxiJ / mxiK / mxiN / mxiL / mxiM / mxiE / mxiD / mxiC / mxiA / spa15 / spa47 / spa13 / spa32 / spa33 / spa24 / spa9 / spa29 / spa40 / spa40 / virA / virA / icsA/virG / nleE / icsP/sopA / ospB / ospC4 / ospC2 / ospD2 / ospD2 / ospD2	1..189871	189871
	-	inside	IScluster/Tn	-	espC / ipaH2.5 / ipaH2.5 / ospE2 / espC / ospC3	85868..121609	35741

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (29-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (19-94)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17736.50 Da
• Isoelectric Point: 7.0382

>T95930 WP_000405248.1 NZ_CP026787:c109951-109469 [Shigella dysenteriae]
MEINVTAPALLTDEHILQPFDCGNEVLSNWLRGRAMKNQMLNASRTFVICLEDTLRVVGYYSLATGSVTHAELGRSLRHN
MPNPVPVVLLGRLAVDVCTQGHGFGKWLLSDAIHRVVNLADQVGIKAVMVHAIDDDARAFYERFGFVQSVVAPNTLFYKV

Nucleotide

• Length: 483 bp

>T95930 NZ_CP026787:c109951-109469 [Shigella dysenteriae]
ATGGAAATAAATGTCACCGCGCCAGCATTGTTGACGGATGAGCATATACTTCAGCCATTTGACTGTGGAAATGAGGTGCT
AAGTAACTGGTTACGTGGTCGGGCTATGAAAAACCAGATGCTCAATGCTTCTCGCACGTTCGTTATTTGCTTGGAGGATA
CTTTACGTGTTGTGGGATACTACTCATTAGCTACTGGTTCCGTCACTCATGCCGAGCTCGGTCGCAGTTTGCGGCATAAC
ATGCCCAATCCTGTCCCTGTTGTTCTGTTAGGGCGTCTGGCTGTTGATGTTTGTACCCAGGGGCATGGTTTCGGTAAATG
GCTACTAAGTGATGCCATTCACCGGGTTGTCAATCTGGCTGATCAGGTTGGCATTAAGGCTGTTATGGTACATGCAATTG
ATGATGACGCCAGAGCATTTTATGAGCGTTTTGGTTTTGTTCAGTCAGTTGTGGCACCTAATACCTTGTTTTATAAAGTC
TGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11737.32 Da
• Isoelectric Point: 4.9167

>AT95930 WP_162147618.1 NZ_CP026787:c110244-109942 [Shigella dysenteriae]
MEFFMSTAASVRKTPREHQINIRATDEERAVIDYAASLVNKNRTDFIMELAYQEAKNIILDQRLFVLDNERYDSFITQLE
APVQNAEGRERLMAVKPEWK

Nucleotide

• Length: 303 bp

>AT95930 NZ_CP026787:c110244-109942 [Shigella dysenteriae]
ATGGAGTTTTTTATGTCTACAGCTGCAAGCGTCCGTAAAACACCGCGAGAACATCAGATTAACATCCGGGCAACTGATGA
GGAACGAGCCGTTATTGATTATGCTGCGAGCTTGGTCAATAAGAACCGTACCGATTTCATCATGGAACTGGCGTACCAGG
AAGCTAAAAACATTATCCTGGATCAACGTTTATTTGTGCTGGATAACGAACGTTATGATTCGTTTATTACGCAACTGGAA
GCTCCTGTGCAGAATGCAGAAGGGCGTGAACGTCTGATGGCGGTAAAGCCTGAATGGAAATAA

Structures

Toxin

• AlphaFold DB: A0A090NWA7