Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | relBE/RHH(antitoxin) |
| Location | 51686..52245 | Replicon | plasmid unnamed |
| Accession | NZ_CP026787 | ||
| Organism | Shigella dysenteriae strain NCTC 9718 | ||
Toxin (Protein)
| Gene name | relE | Uniprot ID | Q327A7 |
| Locus tag | C1P65_RS24265 | Protein ID | WP_000421263.1 |
| Coordinates | 51970..52245 (+) | Length | 92 a.a. |
Antitoxin (Protein)
| Gene name | relB | Uniprot ID | Q327A8 |
| Locus tag | C1P65_RS24260 | Protein ID | WP_001178088.1 |
| Coordinates | 51686..51970 (+) | Length | 95 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| C1P65_RS24205 | 46756..47156 | - | 401 | Protein_57 | transposase | - |
| C1P65_RS26390 | 47221..47361 | - | 141 | Protein_58 | IS3 family transposase | - |
| C1P65_RS24220 | 48223..48723 | - | 501 | Protein_60 | transposase | - |
| C1P65_RS26395 | 48838..49476 | - | 639 | WP_000607008.1 | type III secretion system effector OspI | - |
| C1P65_RS24230 | 49635..50104 | - | 470 | Protein_62 | IS1 family transposase | - |
| C1P65_RS24240 | 50440..50625 | + | 186 | Protein_63 | ATP-binding protein | - |
| C1P65_RS24245 | 50725..50934 | - | 210 | Protein_64 | peptidoglycan-binding protein | - |
| C1P65_RS24260 | 51686..51970 | + | 285 | WP_001178088.1 | ribbon-helix-helix domain-containing protein | Antitoxin |
| C1P65_RS24265 | 51970..52245 | + | 276 | WP_000421263.1 | type II toxin-antitoxin system RelE/ParE family toxin | Toxin |
| C1P65_RS26400 | 52377..52514 | - | 138 | WP_000766699.1 | hypothetical protein | - |
| C1P65_RS24270 | 52606..52971 | - | 366 | WP_000361390.1 | plasmid partitioning/stability family protein | - |
| C1P65_RS24275 | 52974..53542 | - | 569 | Protein_69 | plasmid segregation protein ParM | - |
| C1P65_RS24280 | 53550..54640 | + | 1091 | Protein_70 | IS66 family transposase | - |
| C1P65_RS24285 | 55016..55709 | - | 694 | Protein_71 | IS1 family transposase | - |
| C1P65_RS24290 | 55949..56167 | + | 219 | WP_000813626.1 | type II toxin-antitoxin system antitoxin CcdA | - |
| C1P65_RS24295 | 56169..56474 | + | 306 | WP_001159860.1 | type II toxin-antitoxin system toxin CcdB | - |
| C1P65_RS24300 | 56510..56605 | + | 96 | Protein_74 | transposase domain-containing protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Non-Mobilizable plasmid | - | nleE / icsP/sopA / ospB / ospC2 / ospD2 / ospF / ospD1 / ipgB2 / ipaH4.5 / ipaH7.8 / ospI / ipaH9.8 / ospG / espC / ipaH2.5 / ipaH2.5 / ospE2 / espC / ospC3 / ipaJ / ipaA / ipaD / ipaC / ipaB / ipgC / ipgB1 / ipgA / icsB / ipgD / ipgE / ipgF / mxiG / mxiH / mxiI / mxiJ / mxiK / mxiN / mxiL / mxiM / mxiE / mxiD / mxiC / mxiA / spa15 / spa47 / spa13 / spa32 / spa33 / spa24 / spa9 / spa29 / spa40 / spa40 / virA / virA / icsA/virG / nleE / icsP/sopA / ospB / ospC4 / ospC2 / ospD2 / ospD2 / ospD2 | 1..189871 | 189871 | |
| - | inside | IScluster/Tn | - | ospI / ipaH9.8 / ospG | 45223..62629 | 17406 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 92 a.a. Molecular weight: 10792.51 Da Isoelectric Point: 9.8135
>T95927 WP_000421263.1 NZ_CP026787:51970-52245 [Shigella dysenteriae]
MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFTGEYEIRYELTGQTIY
VLRLWYTRENR
MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFTGEYEIRYELTGQTIY
VLRLWYTRENR
Download Length: 276 bp
>T95927 NZ_CP026787:51970-52245 [Shigella dysenteriae]
ATGGAACTGAAGTGGACCAGTAAGGCACTTTCTGATTTGGCGCGGTTATATGATTTTCTAGTGCTGGCCAGTAAACCTGC
TGCCGCCAGAACGGTGCAGTCCCTGACACAGGCGCCGGTCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTACTGGCGAGTACGAAATCCGTTACGAACTTACTGGCCAGACTATTTAT
GTATTGCGCCTGTGGTACACACGAGAGAACAGGTAG
ATGGAACTGAAGTGGACCAGTAAGGCACTTTCTGATTTGGCGCGGTTATATGATTTTCTAGTGCTGGCCAGTAAACCTGC
TGCCGCCAGAACGGTGCAGTCCCTGACACAGGCGCCGGTCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTACTGGCGAGTACGAAATCCGTTACGAACTTACTGGCCAGACTATTTAT
GTATTGCGCCTGTGGTACACACGAGAGAACAGGTAG
Antitoxin
Download Length: 95 a.a. Molecular weight: 10497.93 Da Isoelectric Point: 6.2150
>AT95927 WP_001178088.1 NZ_CP026787:51686-51970 [Shigella dysenteriae]
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDHPLPVPR
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDHPLPVPR
Download Length: 285 bp
>AT95927 NZ_CP026787:51686-51970 [Shigella dysenteriae]
ATGCAAATGAAAAACAATACCGCACAAGCAACAAAAGTAATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGCTCCCGGGGCTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAAGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATCGACCATCAGGCTGTACAGGCCTGG
GCGGACAGCCTCAGTACTGACCATCCGTTACCGGTGCCACGCTGA
ATGCAAATGAAAAACAATACCGCACAAGCAACAAAAGTAATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGCTCCCGGGGCTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAAGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATCGACCATCAGGCTGTACAGGCCTGG
GCGGACAGCCTCAGTACTGACCATCCGTTACCGGTGCCACGCTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|