Detailed information of TA system TA095925

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 3267679..3268262
• Replicon: chromosome
• Accession: NZ_CP026786
• Organism: Shigella dysenteriae strain NCTC 9718

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: S1EZP4
• Locus tag: C1P65_RS17915
• Protein ID: WP_000254738.1
• Coordinates: 3267927..3268262 (+)
• Length: 112 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: S1P3W5
• Locus tag: C1P65_RS17910
• Protein ID: WP_000581937.1
• Coordinates: 3267679..3267927 (+)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C1P65_RS17900	3264018..3265319	+	1302	WP_000046834.1	23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD	-
C1P65_RS17905	3265367..3267601	+	2235	WP_000226815.1	GTP pyrophosphokinase	-
C1P65_RS17910	3267679..3267927	+	249	WP_000581937.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
C1P65_RS17915	3267927..3268262	+	336	WP_000254738.1	endoribonuclease MazF	Toxin
C1P65_RS17920	3268333..3269124	+	792	WP_001071643.1	nucleoside triphosphate pyrophosphohydrolase	-
C1P65_RS17925	3269347..3270984	+	1638	WP_000210878.1	CTP synthase (glutamine hydrolyzing)	-
C1P65_RS17930	3271072..3272370	+	1299	WP_000036723.1	phosphopyruvate hydratase	-
C1P65_RS17935	3272430..3272780	-	351	Protein_3340	YgcG family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	3265367..3323559	58192

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (10-109)

Antitoxin

• SpoVT-AbrB domain (7-51)

Sequences

Toxin

Protein

• Length: 112 a.a.
• Molecular weight: 12098.04 Da
• Isoelectric Point: 8.2618

>T95925 WP_000254738.1 NZ_CP026786:3267927-3268262 [Shigella dysenteriae]
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Nucleotide

• Length: 336 bp

>T95925 NZ_CP026786:3267927-3268262 [Shigella dysenteriae]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCCGG
ACATCGTCCGGCTGTTGTCCTGAGTCCGTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9355.74 Da
• Isoelectric Point: 4.4665

>AT95925 WP_000581937.1 NZ_CP026786:3267679-3267927 [Shigella dysenteriae]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Nucleotide

• Length: 249 bp

>AT95925 NZ_CP026786:3267679-3267927 [Shigella dysenteriae]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACTTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Structures

Toxin

• PDB: 1UB4
• PDB: 5CQX
• PDB: 5CK9
• PDB: 5CKH
• PDB: 5CO7
• PDB: 3NFC
• PDB: 5CR2
• PDB: 5CKB
• PDB: 5CQY
• PDB: 5CKD
• PDB: 5CKE
• PDB: 5CKF

Antitoxin

• PDB: 1UB4
• PDB: 5CQX
• PDB: 5CQY
• PDB: 1MVF
• PDB: 2MRN
• PDB: 2MRU
• AlphaFold DB: A0A7U9LMB4