Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 46007..46632 Replicon plasmid unnamed
Accession NZ_CP026785
Organism Shigella dysenteriae strain 80-547

Toxin (Protein)


Gene name vapC Uniprot ID A0A090N9J2
Locus tag C5B79_RS24255 Protein ID WP_000911312.1
Coordinates 46234..46632 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID Q326X5
Locus tag C5B79_RS24250 Protein ID WP_000450529.1
Coordinates 46007..46234 (+) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C5B79_RS24245 45506..45925 - 420 WP_011379018.1 conjugative relaxase -
C5B79_RS24250 46007..46234 + 228 WP_000450529.1 toxin-antitoxin system antitoxin VapB Antitoxin
C5B79_RS24255 46234..46632 + 399 WP_000911312.1 tRNA(fMet)-specific endonuclease VapC Toxin
C5B79_RS24260 46641..48423 - 1783 Protein_51 type IV conjugative transfer system coupling protein TraD -
C5B79_RS24265 48489..49645 + 1157 WP_134806340.1 IS3 family transposase -
C5B79_RS24270 49686..50444 - 759 Protein_53 IS4 family transposase -
C5B79_RS24275 50617..51039 + 423 Protein_54 hypothetical protein -
C5B79_RS26785 51184..51315 - 132 Protein_55 transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - mxiG / mxiG / ipgF / ipgE / ipgD / icsB / icsB / ipgA / ipgB1 / ipgB1 / ipgB1 / ipaB / ipaB / ipaC / ipaD / ipaA / ipaJ / ospC3 / ospG / ipaH9.8 / ospI / ospC2 / ospD3/senA / ospC1 / ospE2 / ipaH1.4 / ipaH7.8 / ipaH4.5 / ipgB2 / ospD1 / ospF / ospD2 / ospC2 / ospC4 / ospB / icsP/sopA / nleE / icsA/virG / icsA/virG / virA / virA / spa40 / spa29 / spa9 / spa24 / spa33 / spa32 / spa13 / spa47 / spa15 / mxiA / mxiC / mxiD / mxiE / mxiM / mxiL / mxiN / mxiK / mxiJ / mxiI / mxiH / mxiG / ipgF / ipgE / ipgD / icsB / ipgA / ipgB1 / ipgC / ipaB / ipaC / ipaD / ipaA / ipaJ 1..204250 204250


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-125)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14869.20 Da        Isoelectric Point: 8.5264

>T95907 WP_000911312.1 NZ_CP026785:46234-46632 [Shigella dysenteriae]
MLKFMLDTNICIFTIKNKPASVRERFNLNQGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAAIHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIIVTNNTREFERVGGLRIEDWS

Download         Length: 399 bp

>T95907 NZ_CP026785:46234-46632 [Shigella dysenteriae]
ATGCTGAAGTTTATGCTCGATACCAACATCTGTATTTTTACGATAAAGAACAAACCCGCCAGCGTCAGGGAGCGTTTTAA
CCTTAACCAGGGGAGAATGTGTATCAGTTCGGTCACCCTGATGGAGCTGATATATGGTGCAGAAAAAAGCCAGATGCCTG
AACGTAATCTCGCTGTGATCGAGGGATTTGTTTCCCGCATTGATGTTCTGGATTACGACGCCGCTGCAGCCATACACACC
GGCCAGATAAGAGCAGAACTTGCCCGTCAGGGACGCCCTGTCGGGCCATTTGATCAAATGATCGCAGGTCATGCCCGCAG
TCGGGGGCTGATTATTGTGACTAACAACACCCGGGAATTTGAACGTGTGGGCGGCCTGAGAATTGAAGACTGGAGCTGA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8593.63 Da        Isoelectric Point: 4.5680

>AT95907 WP_000450529.1 NZ_CP026785:46007-46234 [Shigella dysenteriae]
METTVFLSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAGETWDEWFDGHSVSADFMDNREQPDMQERESF

Download         Length: 228 bp

>AT95907 NZ_CP026785:46007-46234 [Shigella dysenteriae]
ATGGAAACCACCGTATTTCTCAGCAACCGCAGCCAGGCGGTCAGACTGCCAAAAGCGGTTGCATTGCCGGAAAACGTAAA
ACGCGTTGAAGTGATTGCTGTCGGACGAACCCGAATCATTACGCCAGCCGGAGAGACATGGGACGAATGGTTCGACGGAC
ACAGCGTCAGCGCCGATTTTATGGATAACAGGGAACAGCCCGACATGCAGGAAAGGGAGTCATTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090N9J2

Antitoxin

Source ID Structure
AlphaFold DB A0A090NVK5

References