Detailed information of TA system TA095906

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 28634..29409
• Replicon: plasmid unnamed
• Accession: NZ_CP026785
• Organism: Shigella dysenteriae strain 80-547

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: Q326V1
• Locus tag: C5B79_RS24145
• Protein ID: WP_000405248.1
• Coordinates: 28927..29409 (+)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: -
• Locus tag: C5B79_RS24140
• Protein ID: WP_162147618.1
• Coordinates: 28634..28936 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C5B79_RS24110	25041..25214	+	174	WP_011379008.1	transposase	-
C5B79_RS24115	25351..25875	+	525	Protein_24	transposase	-
C5B79_RS24125	25958..26230	+	273	WP_000019161.1	hypothetical protein	-
C5B79_RS24130	26211..27353	+	1143	Protein_26	IS91 family transposase	-
C5B79_RS24135	27486..28220	+	735	Protein_27	IS21-like element IS100kyp family helper ATPase IstB	-
C5B79_RS24140	28634..28936	+	303	WP_162147618.1	DUF1778 domain-containing protein	Antitoxin
C5B79_RS24145	28927..29409	+	483	WP_000405248.1	GNAT family N-acetyltransferase	Toxin
C5B79_RS24150	29477..30000	+	524	Protein_30	transposase	-
C5B79_RS26775	30043..30726	-	684	Protein_31	IS3 family transposase	-
C5B79_RS26780	30709..31506	-	798	Protein_32	T3SS effector E3 ubiquitin-protein ligase IpaH1.4	-
C5B79_RS24160	31581..31853	+	273	WP_000019161.1	hypothetical protein	-
C5B79_RS24165	31834..33042	+	1209	WP_000198558.1	IS91 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	mxiG / mxiG / ipgF / ipgE / ipgD / icsB / icsB / ipgA / ipgB1 / ipgB1 / ipgB1 / ipaB / ipaB / ipaC / ipaD / ipaA / ipaJ / ospC3 / ospG / ipaH9.8 / ospI / ospC2 / ospD3/senA / ospC1 / ospE2 / ipaH1.4 / ipaH7.8 / ipaH4.5 / ipgB2 / ospD1 / ospF / ospD2 / ospC2 / ospC4 / ospB / icsP/sopA / nleE / icsA/virG / icsA/virG / virA / virA / spa40 / spa29 / spa9 / spa24 / spa33 / spa32 / spa13 / spa47 / spa15 / mxiA / mxiC / mxiD / mxiE / mxiM / mxiL / mxiN / mxiK / mxiJ / mxiI / mxiH / mxiG / ipgF / ipgE / ipgD / icsB / ipgA / ipgB1 / ipgC / ipaB / ipaC / ipaD / ipaA / ipaJ	1..204250	204250
	-	inside	IScluster/Tn	-	ospC3	17267..36632	19365

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (29-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (19-94)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17736.50 Da
• Isoelectric Point: 7.0382

>T95906 WP_000405248.1 NZ_CP026785:28927-29409 [Shigella dysenteriae]
MEINVTAPALLTDEHILQPFDCGNEVLSNWLRGRAMKNQMLNASRTFVICLEDTLRVVGYYSLATGSVTHAELGRSLRHN
MPNPVPVVLLGRLAVDVCTQGHGFGKWLLSDAIHRVVNLADQVGIKAVMVHAIDDDARAFYERFGFVQSVVAPNTLFYKV

Nucleotide

• Length: 483 bp

>T95906 NZ_CP026785:28927-29409 [Shigella dysenteriae]
ATGGAAATAAATGTCACCGCGCCAGCATTGTTGACGGATGAGCATATACTTCAGCCATTTGACTGTGGAAATGAGGTGCT
AAGTAACTGGTTACGTGGTCGGGCTATGAAAAACCAGATGCTCAATGCTTCTCGCACGTTCGTTATTTGCTTGGAGGATA
CTTTACGTGTTGTGGGATACTACTCATTAGCTACTGGTTCCGTCACTCATGCCGAGCTCGGTCGCAGTTTGCGGCATAAC
ATGCCCAATCCTGTCCCTGTTGTTCTGTTAGGGCGTCTGGCTGTTGATGTTTGTACCCAGGGGCATGGTTTCGGTAAATG
GCTACTAAGTGATGCCATTCACCGGGTTGTCAATCTGGCTGATCAGGTTGGCATTAAGGCTGTTATGGTACATGCAATTG
ATGATGACGCCAGAGCATTTTATGAGCGTTTTGGTTTTGTTCAGTCAGTTGTGGCACCTAATACCTTGTTTTATAAAGTC
TGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11737.32 Da
• Isoelectric Point: 4.9167

>AT95906 WP_162147618.1 NZ_CP026785:28634-28936 [Shigella dysenteriae]
MEFFMSTAASVRKTPREHQINIRATDEERAVIDYAASLVNKNRTDFIMELAYQEAKNIILDQRLFVLDNERYDSFITQLE
APVQNAEGRERLMAVKPEWK

Nucleotide

• Length: 303 bp

>AT95906 NZ_CP026785:28634-28936 [Shigella dysenteriae]
ATGGAGTTTTTTATGTCTACAGCTGCAAGCGTCCGTAAAACACCGCGAGAACATCAGATTAACATCCGGGCAACTGATGA
GGAACGAGCCGTTATTGATTATGCTGCGAGCTTGGTCAATAAGAACCGTACCGATTTCATCATGGAACTGGCGTACCAGG
AAGCTAAAAACATTATCCTGGATCAACGTTTATTTGTGCTGGATAACGAACGTTATGATTCGTTTATTACGCAACTGGAA
GCTCCTGTGCAGAATGCAGAAGGGCGTGAACGTCTGATGGCGGTAAAGCCTGAATGGAAATAA

Structures

Toxin

• AlphaFold DB: A0A090NWA7