Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 2264759..2265309 Replicon chromosome
Accession NZ_CP026782
Organism Shigella dysenteriae strain 08-3380

Toxin (Protein)


Gene name ccdB Uniprot ID Q32K46
Locus tag C1P64_RS12520 Protein ID WP_001160974.1
Coordinates 2264995..2265309 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID D3H431
Locus tag C1P64_RS12515 Protein ID WP_000125565.1
Coordinates 2264759..2264992 (+) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C1P64_RS12490 2260025..2260312 + 288 WP_000203741.1 ferredoxin-like protein FixX -
C1P64_RS12495 2260370..2261701 + 1332 Protein_2274 MFS transporter -
C1P64_RS12500 2261812..2262342 + 531 WP_000600719.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
C1P64_RS12505 2262335..2264002 + 1668 WP_000377138.1 glutathione-regulated potassium-efflux system protein -
C1P64_RS12510 2264194..2264673 + 480 WP_005020907.1 type 3 dihydrofolate reductase -
C1P64_RS12515 2264759..2264992 + 234 WP_000125565.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
C1P64_RS12520 2264995..2265309 + 315 WP_001160974.1 CcdB family protein Toxin
C1P64_RS12525 2265306..2266154 - 849 WP_000257202.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
C1P64_RS12530 2266161..2266538 - 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
C1P64_RS12535 2266541..2267362 - 822 WP_001065369.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
C1P64_RS12540 2267359..2268348 - 990 WP_000241239.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
C1P64_RS12545 2268348..2269634 - 1287 WP_000800471.1 peptidylprolyl isomerase SurA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-103)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11737.84 Da        Isoelectric Point: 9.8517

>T95871 WP_001160974.1 NZ_CP026782:2264995-2265309 [Shigella dysenteriae]
MQFTVYRSRSRNAAFPFVIDVTSDIIGVINRRIVIPLTPIERFIRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Download         Length: 315 bp

>T95871 NZ_CP026782:2264995-2265309 [Shigella dysenteriae]
ATGCAATTTACGGTATACCGCAGTCGCAGCAGGAACGCCGCGTTTCCCTTTGTTATCGATGTTACCAGCGACATTATTGG
TGTGATTAATCGCCGTATTGTTATTCCATTAACACCTATTGAACGATTTATCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGGTAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTCCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAAGGAGCATTAGATTTTATGCTCGACGGGATTTAA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8599.64 Da        Isoelectric Point: 6.9791

>AT95871 WP_000125565.1 NZ_CP026782:2264759-2264992 [Shigella dysenteriae]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRRF

Download         Length: 234 bp

>AT95871 NZ_CP026782:2264759-2264992 [Shigella dysenteriae]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGCGCTTCTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LRR3

Antitoxin

Source ID Structure
AlphaFold DB A0A090NGC7

References