Detailed information of TA system TA095865

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 2871..3430
• Replicon: plasmid unnamed
• Accession: NZ_CP026781
• Organism: Shigella dysenteriae strain 53-3937

Toxin (Protein)

• Gene name: relE
• Uniprot ID: Q327A7
• Locus tag: C1P63_RS23975
• Protein ID: WP_000421263.1
• Coordinates: 2871..3146 (-)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: Q327A8
• Locus tag: C1P63_RS23980
• Protein ID: WP_001178088.1
• Coordinates: 3146..3430 (-)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C1P63_RS23955	1..100	+	100	Protein_0	IS1 family transposase	-
C1P63_RS23960	476..1566	-	1091	Protein_1	IS66 family transposase	-
C1P63_RS23965	1574..2142	+	569	Protein_2	plasmid segregation protein ParM	-
C1P63_RS23970	2145..2510	+	366	WP_000361391.1	plasmid partitioning/stability family protein	-
C1P63_RS25595	2602..2739	+	138	WP_000766699.1	hypothetical protein	-
C1P63_RS23975	2871..3146	-	276	WP_000421263.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C1P63_RS23980	3146..3430	-	285	WP_001178088.1	ribbon-helix-helix domain-containing protein	Antitoxin
C1P63_RS23995	4182..4391	+	210	Protein_7	peptidoglycan-binding protein	-
C1P63_RS24000	4491..4676	-	186	Protein_8	ATP-binding protein	-
C1P63_RS24010	5012..5481	+	470	Protein_9	IS1 family transposase	-
C1P63_RS25600	5640..6278	+	639	WP_000607008.1	type III secretion system effector OspI	-
C1P63_RS24020	6393..6926	+	534	Protein_11	transposase	-
C1P63_RS25605	6981..7121	+	141	Protein_12	IS3 family transposase	-
C1P63_RS24025	7186..7586	+	401	Protein_13	transposase	-
C1P63_RS24030	7642..8339	+	698	Protein_14	IS1 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	ospI / ospC2 / ospD3/senA / ospC1 / ospG / ipaH9.8	1..67064	67064
	-	inside	IScluster/Tn	-	ospI / ospC2	476..24024	23548

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (14-51)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10792.51 Da
• Isoelectric Point: 9.8135

>T95865 WP_000421263.1 NZ_CP026781:c3146-2871 [Shigella dysenteriae]
MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFTGEYEIRYELTGQTIY
VLRLWYTRENR

Nucleotide

• Length: 276 bp

>T95865 NZ_CP026781:c3146-2871 [Shigella dysenteriae]
ATGGAACTGAAGTGGACCAGTAAGGCACTTTCTGATTTGGCGCGGTTATATGATTTTCTAGTGCTGGCCAGTAAACCTGC
TGCCGCCAGAACGGTGCAGTCCCTGACACAGGCGCCGGTCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTACTGGCGAGTACGAAATCCGTTACGAACTTACTGGCCAGACTATTTAT
GTATTGCGCCTGTGGTACACACGAGAGAACAGGTAG

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10497.93 Da
• Isoelectric Point: 6.2150

>AT95865 WP_001178088.1 NZ_CP026781:c3430-3146 [Shigella dysenteriae]
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDHPLPVPR

Nucleotide

• Length: 285 bp

>AT95865 NZ_CP026781:c3430-3146 [Shigella dysenteriae]
ATGCAAATGAAAAACAATACCGCACAAGCAACAAAAGTAATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGCTCCCGGGGCTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAAGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATCGACCATCAGGCTGTACAGGCCTGG
GCGGACAGCCTCAGTACTGACCATCCGTTACCGGTGCCACGCTGA

Structures

Toxin

• AlphaFold DB: Q327A7

Antitoxin

• AlphaFold DB: Q327A8