95847

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 180729..181354 Replicon plasmid unnamed2
Accession NZ_CP026779
Organism Shigella dysenteriae strain 69-3818

Toxin (Protein)


Gene name vapC Uniprot ID A0A090N9J2
Locus tag C5C01_RS26075 Protein ID WP_000911312.1
Coordinates 180729..181127 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID Q326X5
Locus tag C5C01_RS26080 Protein ID WP_000450529.1
Coordinates 181127..181354 (-) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C5C01_RS28030 176048..176179 + 132 Protein_209 transposase -
C5C01_RS26055 176324..176746 - 423 Protein_210 hypothetical protein -
C5C01_RS26060 176919..177692 + 774 Protein_211 IS4 family transposase -
C5C01_RS26070 178939..180720 + 1782 Protein_213 type IV conjugative transfer system coupling protein TraD -
C5C01_RS26075 180729..181127 - 399 WP_000911312.1 tRNA(fMet)-specific endonuclease VapC Toxin
C5C01_RS26080 181127..181354 - 228 WP_000450529.1 toxin-antitoxin system antitoxin VapB Antitoxin
C5C01_RS26085 181531..181854 + 324 WP_171764096.1 relaxase domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - ospE2 / ipaH2.5 / ospC3 / ipaJ / ipaA / ipaA / ipaD / ipaC / ipaB / ipgC / ipgB1 / ipgA / icsB / ipgD / ipgE / ipgF / mxiG / mxiH / mxiI / mxiJ / mxiK / mxiN / mxiL / mxiM / mxiE / mxiD / mxiD / mxiC / mxiA / spa15 / spa47 / spa13 / spa32 / spa33 / spa24 / spa9 / spa29 / spa40 / spa40 / virA / virA / icsA/virG / nleE / icsP/sopA / ospB / ospC2 / ospD2 / ospF / ospD1 / ipgB2 / ipaH4.5 / ipaH7.8 / ipaH1.4 / ospE2 / ospC1 / ospD3/senA / ospD3/senA / ospC2 / ospI / ipaH9.8 / ospG / espC 1..202417 202417


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-125)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14869.20 Da        Isoelectric Point: 8.5264

>T95847 WP_000911312.1 NZ_CP026779:c181127-180729 [Shigella dysenteriae]
MLKFMLDTNICIFTIKNKPASVRERFNLNQGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAAIHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIIVTNNTREFERVGGLRIEDWS

Download         Length: 399 bp

>T95847 NZ_CP026779:c181127-180729 [Shigella dysenteriae]
ATGCTGAAGTTTATGCTCGATACCAACATCTGTATTTTTACGATAAAGAACAAACCCGCCAGCGTCAGGGAGCGTTTTAA
CCTTAACCAGGGGAGAATGTGTATCAGTTCGGTCACCCTGATGGAGCTGATATATGGTGCAGAAAAAAGCCAGATGCCTG
AACGTAATCTCGCTGTGATCGAGGGATTTGTTTCCCGCATTGATGTTCTGGATTACGACGCCGCTGCAGCCATACACACC
GGCCAGATAAGAGCAGAACTTGCCCGTCAGGGACGCCCTGTCGGGCCATTTGATCAAATGATCGCAGGTCATGCCCGCAG
TCGGGGGCTGATTATTGTGACTAACAACACCCGGGAATTTGAACGTGTGGGCGGCCTGAGAATTGAAGACTGGAGCTGA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8593.63 Da        Isoelectric Point: 4.5680

>AT95847 WP_000450529.1 NZ_CP026779:c181354-181127 [Shigella dysenteriae]
METTVFLSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAGETWDEWFDGHSVSADFMDNREQPDMQERESF

Download         Length: 228 bp

>AT95847 NZ_CP026779:c181354-181127 [Shigella dysenteriae]
ATGGAAACCACCGTATTTCTCAGCAACCGCAGCCAGGCGGTCAGACTGCCAAAAGCGGTTGCATTGCCGGAAAACGTAAA
ACGCGTTGAAGTGATTGCTGTCGGACGAACCCGAATCATTACGCCAGCCGGAGAGACATGGGACGAATGGTTCGACGGAC
ACAGCGTCAGCGCCGATTTTATGGATAACAGGGAACAGCCCGACATGCAGGAAAGGGAGTCATTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090N9J2

Antitoxin

Source ID Structure
AlphaFold DB A0A090NVK5

References