Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | relBE/RHH(antitoxin) |
| Location | 154019..154578 | Replicon | plasmid unnamed2 |
| Accession | NZ_CP026779 | ||
| Organism | Shigella dysenteriae strain 69-3818 | ||
Toxin (Protein)
| Gene name | relE | Uniprot ID | Q327A7 |
| Locus tag | C5C01_RS25920 | Protein ID | WP_000421263.1 |
| Coordinates | 154303..154578 (+) | Length | 92 a.a. |
Antitoxin (Protein)
| Gene name | relB | Uniprot ID | Q327A8 |
| Locus tag | C5C01_RS25915 | Protein ID | WP_001178088.1 |
| Coordinates | 154019..154303 (+) | Length | 95 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| C5C01_RS25860 | 149089..149489 | - | 401 | Protein_173 | transposase | - |
| C5C01_RS27845 | 149554..149694 | - | 141 | Protein_174 | IS3 family transposase | - |
| C5C01_RS25875 | 150556..151056 | - | 501 | Protein_176 | transposase | - |
| C5C01_RS27850 | 151171..151809 | - | 639 | WP_000607008.1 | type III secretion system effector OspI | - |
| C5C01_RS25885 | 151968..152437 | - | 470 | Protein_178 | IS1 family transposase | - |
| C5C01_RS25895 | 152773..152958 | + | 186 | Protein_179 | ATP-binding protein | - |
| C5C01_RS25900 | 153058..153267 | - | 210 | Protein_180 | peptidoglycan-binding protein | - |
| C5C01_RS25915 | 154019..154303 | + | 285 | WP_001178088.1 | ribbon-helix-helix domain-containing protein | Antitoxin |
| C5C01_RS25920 | 154303..154578 | + | 276 | WP_000421263.1 | type II toxin-antitoxin system RelE/ParE family toxin | Toxin |
| C5C01_RS27855 | 154710..154847 | - | 138 | WP_000766699.1 | hypothetical protein | - |
| C5C01_RS25925 | 154939..155304 | - | 366 | WP_000361390.1 | plasmid partitioning/stability family protein | - |
| C5C01_RS25930 | 155307..155875 | - | 569 | Protein_185 | plasmid segregation protein ParM | - |
| C5C01_RS25935 | 155883..156973 | + | 1091 | Protein_186 | IS66 family transposase | - |
| C5C01_RS25940 | 157200..158350 | + | 1151 | Protein_187 | IS3 family transposase | - |
| C5C01_RS25945 | 158617..159309 | - | 693 | Protein_188 | IS1 family transposase | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Non-Mobilizable plasmid | - | ospE2 / ipaH2.5 / ospC3 / ipaJ / ipaA / ipaA / ipaD / ipaC / ipaB / ipgC / ipgB1 / ipgA / icsB / ipgD / ipgE / ipgF / mxiG / mxiH / mxiI / mxiJ / mxiK / mxiN / mxiL / mxiM / mxiE / mxiD / mxiD / mxiC / mxiA / spa15 / spa47 / spa13 / spa32 / spa33 / spa24 / spa9 / spa29 / spa40 / spa40 / virA / virA / icsA/virG / nleE / icsP/sopA / ospB / ospC2 / ospD2 / ospF / ospD1 / ipgB2 / ipaH4.5 / ipaH7.8 / ipaH1.4 / ospE2 / ospC1 / ospD3/senA / ospD3/senA / ospC2 / ospI / ipaH9.8 / ospG / espC | 1..202417 | 202417 | |
| - | inside | IScluster/Tn | - | ipaH1.4 / ospE2 / ospC1 / ospD3/senA / ospD3/senA / ospC2 / ospI / ipaH9.8 / ospG | 119617..168065 | 48448 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 92 a.a. Molecular weight: 10792.51 Da Isoelectric Point: 9.8135
>T95845 WP_000421263.1 NZ_CP026779:154303-154578 [Shigella dysenteriae]
MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFTGEYEIRYELTGQTIY
VLRLWYTRENR
MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFTGEYEIRYELTGQTIY
VLRLWYTRENR
Download Length: 276 bp
>T95845 NZ_CP026779:154303-154578 [Shigella dysenteriae]
ATGGAACTGAAGTGGACCAGTAAGGCACTTTCTGATTTGGCGCGGTTATATGATTTTCTAGTGCTGGCCAGTAAACCTGC
TGCCGCCAGAACGGTGCAGTCCCTGACACAGGCGCCGGTCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTACTGGCGAGTACGAAATCCGTTACGAACTTACTGGCCAGACTATTTAT
GTATTGCGCCTGTGGTACACACGAGAGAACAGGTAG
ATGGAACTGAAGTGGACCAGTAAGGCACTTTCTGATTTGGCGCGGTTATATGATTTTCTAGTGCTGGCCAGTAAACCTGC
TGCCGCCAGAACGGTGCAGTCCCTGACACAGGCGCCGGTCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTACTGGCGAGTACGAAATCCGTTACGAACTTACTGGCCAGACTATTTAT
GTATTGCGCCTGTGGTACACACGAGAGAACAGGTAG
Antitoxin
Download Length: 95 a.a. Molecular weight: 10497.93 Da Isoelectric Point: 6.2150
>AT95845 WP_001178088.1 NZ_CP026779:154019-154303 [Shigella dysenteriae]
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDHPLPVPR
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDHPLPVPR
Download Length: 285 bp
>AT95845 NZ_CP026779:154019-154303 [Shigella dysenteriae]
ATGCAAATGAAAAACAATACCGCACAAGCAACAAAAGTAATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGCTCCCGGGGCTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAAGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATCGACCATCAGGCTGTACAGGCCTGG
GCGGACAGCCTCAGTACTGACCATCCGTTACCGGTGCCACGCTGA
ATGCAAATGAAAAACAATACCGCACAAGCAACAAAAGTAATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGCTCCCGGGGCTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAAGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATCGACCATCAGGCTGTACAGGCCTGG
GCGGACAGCCTCAGTACTGACCATCCGTTACCGGTGCCACGCTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|