Detailed information of TA system TA095844

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 12224..12999
• Replicon: plasmid unnamed2
• Accession: NZ_CP026779
• Organism: Shigella dysenteriae strain 69-3818

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: Q326V1
• Locus tag: C5C01_RS24965
• Protein ID: WP_000405248.1
• Coordinates: 12224..12706 (-)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: -
• Locus tag: C5C01_RS24970
• Protein ID: WP_162147618.1
• Coordinates: 12697..12999 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C5C01_RS24945	8591..9799	-	1209	WP_119178644.1	IS91 family transposase	-
C5C01_RS24950	9780..10052	-	273	WP_000019161.1	hypothetical protein	-
C5C01_RS27995	10127..10924	+	798	Protein_12	T3SS effector E3 ubiquitin-protein ligase IpaH1.4	-
C5C01_RS28000	10907..11590	+	684	Protein_13	IS3 family transposase	-
C5C01_RS24960	11633..12156	-	524	Protein_14	transposase	-
C5C01_RS24965	12224..12706	-	483	WP_000405248.1	GNAT family N-acetyltransferase	Toxin
C5C01_RS24970	12697..12999	-	303	WP_162147618.1	DUF1778 domain-containing protein	Antitoxin
C5C01_RS24975	13413..14147	-	735	Protein_17	IS21-like element IS100kyp family helper ATPase IstB	-
C5C01_RS24980	14280..15422	-	1143	Protein_18	IS91 family transposase	-
C5C01_RS24985	15403..15675	-	273	WP_000019161.1	hypothetical protein	-
C5C01_RS24995	15758..16282	-	525	Protein_20	transposase	-
C5C01_RS25000	16419..16592	-	174	WP_011379008.1	transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	ospE2 / ipaH2.5 / ospC3 / ipaJ / ipaA / ipaA / ipaD / ipaC / ipaB / ipgC / ipgB1 / ipgA / icsB / ipgD / ipgE / ipgF / mxiG / mxiH / mxiI / mxiJ / mxiK / mxiN / mxiL / mxiM / mxiE / mxiD / mxiD / mxiC / mxiA / spa15 / spa47 / spa13 / spa32 / spa33 / spa24 / spa9 / spa29 / spa40 / spa40 / virA / virA / icsA/virG / nleE / icsP/sopA / ospB / ospC2 / ospD2 / ospF / ospD1 / ipgB2 / ipaH4.5 / ipaH7.8 / ipaH1.4 / ospE2 / ospC1 / ospD3/senA / ospD3/senA / ospC2 / ospI / ipaH9.8 / ospG / espC	1..202417	202417
	-	inside	IScluster/Tn	-	ospE2 / ipaH2.5 / ospC3	388..24362	23974

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (29-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (19-94)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17736.50 Da
• Isoelectric Point: 7.0382

>T95844 WP_000405248.1 NZ_CP026779:c12706-12224 [Shigella dysenteriae]
MEINVTAPALLTDEHILQPFDCGNEVLSNWLRGRAMKNQMLNASRTFVICLEDTLRVVGYYSLATGSVTHAELGRSLRHN
MPNPVPVVLLGRLAVDVCTQGHGFGKWLLSDAIHRVVNLADQVGIKAVMVHAIDDDARAFYERFGFVQSVVAPNTLFYKV

Nucleotide

• Length: 483 bp

>T95844 NZ_CP026779:c12706-12224 [Shigella dysenteriae]
ATGGAAATAAATGTCACCGCGCCAGCATTGTTGACGGATGAGCATATACTTCAGCCATTTGACTGTGGAAATGAGGTGCT
AAGTAACTGGTTACGTGGTCGGGCTATGAAAAACCAGATGCTCAATGCTTCTCGCACGTTCGTTATTTGCTTGGAGGATA
CTTTACGTGTTGTGGGATACTACTCATTAGCTACTGGTTCCGTCACTCATGCCGAGCTCGGTCGCAGTTTGCGGCATAAC
ATGCCCAATCCTGTCCCTGTTGTTCTGTTAGGGCGTCTGGCTGTTGATGTTTGTACCCAGGGGCATGGTTTCGGTAAATG
GCTACTAAGTGATGCCATTCACCGGGTTGTCAATCTGGCTGATCAGGTTGGCATTAAGGCTGTTATGGTACATGCAATTG
ATGATGACGCCAGAGCATTTTATGAGCGTTTTGGTTTTGTTCAGTCAGTTGTGGCACCTAATACCTTGTTTTATAAAGTC
TGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11737.32 Da
• Isoelectric Point: 4.9167

>AT95844 WP_162147618.1 NZ_CP026779:c12999-12697 [Shigella dysenteriae]
MEFFMSTAASVRKTPREHQINIRATDEERAVIDYAASLVNKNRTDFIMELAYQEAKNIILDQRLFVLDNERYDSFITQLE
APVQNAEGRERLMAVKPEWK

Nucleotide

• Length: 303 bp

>AT95844 NZ_CP026779:c12999-12697 [Shigella dysenteriae]
ATGGAGTTTTTTATGTCTACAGCTGCAAGCGTCCGTAAAACACCGCGAGAACATCAGATTAACATCCGGGCAACTGATGA
GGAACGAGCCGTTATTGATTATGCTGCGAGCTTGGTCAATAAGAACCGTACCGATTTCATCATGGAACTGGCGTACCAGG
AAGCTAAAAACATTATCCTGGATCAACGTTTATTTGTGCTGGATAACGAACGTTATGATTCGTTTATTACGCAACTGGAA
GCTCCTGTGCAGAATGCAGAAGGGCGTGAACGTCTGATGGCGGTAAAGCCTGAATGGAAATAA

Structures

Toxin

• AlphaFold DB: A0A090NWA7