Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | ccdAB/CcdA(antitoxin) |
| Location | 150286..150811 | Replicon | plasmid unnamed |
| Accession | NZ_CP026775 | ||
| Organism | Shigella dysenteriae strain ATCC 13313 | ||
Toxin (Protein)
| Gene name | ccdB | Uniprot ID | Q326Z8 |
| Locus tag | C1P61_RS24920 | Protein ID | WP_001159860.1 |
| Coordinates | 150286..150591 (-) | Length | 102 a.a. |
Antitoxin (Protein)
| Gene name | ccdA | Uniprot ID | Q7BEK0 |
| Locus tag | C1P61_RS24925 | Protein ID | WP_000813626.1 |
| Coordinates | 150593..150811 (-) | Length | 73 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| C1P61_RS26480 | 145540..145824 | - | 285 | Protein_167 | IS66 family insertion sequence element accessory protein TnpB | - |
| C1P61_RS24890 | 146163..146753 | - | 591 | WP_000705601.1 | type III secretion system effector protein kinase OspG | - |
| C1P61_RS26725 | 146782..147008 | - | 227 | Protein_169 | type II toxin-antitoxin system RelE/ParE family toxin | - |
| C1P61_RS24900 | 147005..147274 | - | 270 | WP_000079957.1 | hypothetical protein | - |
| C1P61_RS24905 | 147482..149119 | - | 1638 | WP_000936810.1 | T3SS effector E3 ubiquitin-protein ligase IpaH9.8 | - |
| C1P61_RS24915 | 150155..150250 | - | 96 | Protein_173 | transposase domain-containing protein | - |
| C1P61_RS24920 | 150286..150591 | - | 306 | WP_001159860.1 | type II toxin-antitoxin system toxin CcdB | Toxin |
| C1P61_RS24925 | 150593..150811 | - | 219 | WP_000813626.1 | type II toxin-antitoxin system antitoxin CcdA | Antitoxin |
| C1P61_RS24930 | 151051..151744 | + | 694 | Protein_176 | IS1 family transposase | - |
| C1P61_RS24935 | 152120..153210 | - | 1091 | Protein_177 | IS66 family transposase | - |
| C1P61_RS24940 | 153218..153786 | + | 569 | Protein_178 | plasmid segregation protein ParM | - |
| C1P61_RS24945 | 153789..154154 | + | 366 | WP_000361390.1 | plasmid partitioning/stability family protein | - |
| C1P61_RS26485 | 154246..154383 | + | 138 | WP_000766699.1 | hypothetical protein | - |
| C1P61_RS24950 | 154515..154790 | - | 276 | WP_000421263.1 | type II toxin-antitoxin system RelE/ParE family toxin | - |
| C1P61_RS24955 | 154790..155074 | - | 285 | WP_001178088.1 | ribbon-helix-helix domain-containing protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Non-Mobilizable plasmid | - | ospE2 / ipaH1.4 / ipaH7.8 / ipaH4.5 / ipgB2 / ospD1 / ospF / ospD2 / ospC2 / ospC4 / ospB / icsP/sopA / nleE / icsA/virG / virA / spa40 / spa40 / spa29 / spa9 / spa24 / spa33 / spa32 / spa13 / spa47 / spa15 / mxiA / mxiC / mxiD / mxiE / mxiM / mxiL / mxiN / mxiK / mxiJ / mxiI / mxiH / mxiG / ipgF / ipgE / ipgD / icsB / ipgA / ipgB1 / ipgC / ipaB / ipaC / ipaD / ipaA / ipaJ / ospC3 / ospG / ipaH9.8 / ospI / ospC2 / ospD3/senA / ospC1 | 1..182697 | 182697 | |
| - | inside | IScluster/Tn | - | ospG / ipaH9.8 / ospI / ospC2 | 144131..174332 | 30201 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 102 a.a. Molecular weight: 11736.59 Da Isoelectric Point: 6.4674
>T95801 WP_001159860.1 NZ_CP026775:c150591-150286 [Shigella dysenteriae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPIFVIGEEV
ADLSHRENDIKNAINLMFWGI
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPIFVIGEEV
ADLSHRENDIKNAINLMFWGI
Download Length: 306 bp
>T95801 NZ_CP026775:c150591-150286 [Shigella dysenteriae]
ATGCAGTTTAAGGTTTACGCCTATAAAAGAGAGAGTCGTTATCGTCTGTTTGTGGATGTACAGAGTGACATTATTGACAC
GCCCGGACGACGGATGGTGATCCCCCTGGCCAGTGCCCGCCTGCTGTCAGATAAAGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACTGATATGGCCAGTGTGCCGATCTTCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA
ATGCAGTTTAAGGTTTACGCCTATAAAAGAGAGAGTCGTTATCGTCTGTTTGTGGATGTACAGAGTGACATTATTGACAC
GCCCGGACGACGGATGGTGATCCCCCTGGCCAGTGCCCGCCTGCTGTCAGATAAAGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACTGATATGGCCAGTGTGCCGATCTTCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA
Antitoxin
Download Length: 73 a.a. Molecular weight: 8333.36 Da Isoelectric Point: 6.2838
>AT95801 WP_000813626.1 NZ_CP026775:c150811-150593 [Shigella dysenteriae]
MKQRITVTIDSDSYQLLKSANVNISGLVNTAMQKEARRLRAERWQAENQQGMAEIARFIEMNGSFADENRDW
MKQRITVTIDSDSYQLLKSANVNISGLVNTAMQKEARRLRAERWQAENQQGMAEIARFIEMNGSFADENRDW
Download Length: 219 bp
>AT95801 NZ_CP026775:c150811-150593 [Shigella dysenteriae]
ATGAAGCAGCGTATTACTGTCACTATAGACAGTGACAGCTATCAGTTACTTAAATCCGCAAACGTGAATATCTCAGGCCT
GGTAAATACGGCCATGCAAAAAGAGGCCCGCCGTCTGCGTGCGGAACGCTGGCAGGCTGAAAATCAGCAGGGGATGGCTG
AGATCGCCCGTTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA
ATGAAGCAGCGTATTACTGTCACTATAGACAGTGACAGCTATCAGTTACTTAAATCCGCAAACGTGAATATCTCAGGCCT
GGTAAATACGGCCATGCAAAAAGAGGCCCGCCGTCTGCGTGCGGAACGCTGGCAGGCTGAAAATCAGCAGGGGATGGCTG
AGATCGCCCGTTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A4P7TTN3 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | Q7BEK0 |