Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 130264..130889 Replicon plasmid unnamed
Accession NZ_CP026775
Organism Shigella dysenteriae strain ATCC 13313

Toxin (Protein)


Gene name vapC Uniprot ID A0A090N9J2
Locus tag C1P61_RS24810 Protein ID WP_000911312.1
Coordinates 130491..130889 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID Q326X5
Locus tag C1P61_RS24805 Protein ID WP_000450529.1
Coordinates 130264..130491 (+) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C1P61_RS24800 129763..130182 - 420 WP_011379018.1 conjugative relaxase -
C1P61_RS24805 130264..130491 + 228 WP_000450529.1 toxin-antitoxin system antitoxin VapB Antitoxin
C1P61_RS24810 130491..130889 + 399 WP_000911312.1 tRNA(fMet)-specific endonuclease VapC Toxin
C1P61_RS24815 130898..132679 - 1782 Protein_152 type IV conjugative transfer system coupling protein TraD -
C1P61_RS24820 132745..133901 + 1157 WP_134801811.1 IS3-like element IS600 family transposase -
C1P61_RS24825 133942..134700 - 759 Protein_154 IS4 family transposase -
C1P61_RS24830 134873..135295 + 423 Protein_155 hypothetical protein -
C1P61_RS26720 135440..135571 - 132 Protein_156 transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - ospE2 / ipaH1.4 / ipaH7.8 / ipaH4.5 / ipgB2 / ospD1 / ospF / ospD2 / ospC2 / ospC4 / ospB / icsP/sopA / nleE / icsA/virG / virA / spa40 / spa40 / spa29 / spa9 / spa24 / spa33 / spa32 / spa13 / spa47 / spa15 / mxiA / mxiC / mxiD / mxiE / mxiM / mxiL / mxiN / mxiK / mxiJ / mxiI / mxiH / mxiG / ipgF / ipgE / ipgD / icsB / ipgA / ipgB1 / ipgC / ipaB / ipaC / ipaD / ipaA / ipaJ / ospC3 / ospG / ipaH9.8 / ospI / ospC2 / ospD3/senA / ospC1 1..182697 182697


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-125)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14869.20 Da        Isoelectric Point: 8.5264

>T95800 WP_000911312.1 NZ_CP026775:130491-130889 [Shigella dysenteriae]
MLKFMLDTNICIFTIKNKPASVRERFNLNQGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAAIHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIIVTNNTREFERVGGLRIEDWS

Download         Length: 399 bp

>T95800 NZ_CP026775:130491-130889 [Shigella dysenteriae]
ATGCTGAAGTTTATGCTCGATACCAACATCTGTATTTTTACGATAAAGAACAAACCCGCCAGCGTCAGGGAGCGTTTTAA
CCTTAACCAGGGGAGAATGTGTATCAGTTCGGTCACCCTGATGGAGCTGATATATGGTGCAGAAAAAAGCCAGATGCCTG
AACGTAATCTCGCTGTGATCGAGGGATTTGTTTCCCGCATTGATGTTCTGGATTACGACGCCGCTGCAGCCATACACACC
GGCCAGATAAGAGCAGAACTTGCCCGTCAGGGACGCCCTGTCGGGCCATTTGATCAAATGATCGCAGGTCATGCCCGCAG
TCGGGGGCTGATTATTGTGACTAACAACACCCGGGAATTTGAACGTGTGGGCGGCCTGAGAATTGAAGACTGGAGCTGA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8593.63 Da        Isoelectric Point: 4.5680

>AT95800 WP_000450529.1 NZ_CP026775:130264-130491 [Shigella dysenteriae]
METTVFLSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAGETWDEWFDGHSVSADFMDNREQPDMQERESF

Download         Length: 228 bp

>AT95800 NZ_CP026775:130264-130491 [Shigella dysenteriae]
ATGGAAACCACCGTATTTCTCAGCAACCGCAGCCAGGCGGTCAGACTGCCAAAAGCGGTTGCATTGCCGGAAAACGTAAA
ACGCGTTGAAGTGATTGCTGTCGGACGAACCCGAATCATTACGCCAGCCGGAGAGACATGGGACGAATGGTTCGACGGAC
ACAGCGTCAGCGCCGATTTTATGGATAACAGGGAACAGCCCGACATGCAGGAAAGGGAGTCATTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090N9J2

Antitoxin

Source ID Structure
AlphaFold DB A0A090NVK5

References