Detailed information of TA system TA095799

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 112891..113666
• Replicon: plasmid unnamed
• Accession: NZ_CP026775
• Organism: Shigella dysenteriae strain ATCC 13313

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: Q326V1
• Locus tag: C1P61_RS24700
• Protein ID: WP_000405248.1
• Coordinates: 113184..113666 (+)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: -
• Locus tag: C1P61_RS24695
• Protein ID: WP_162147618.1
• Coordinates: 112891..113193 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C1P61_RS24665	109298..109471	+	174	WP_011379008.1	transposase	-
C1P61_RS24670	109608..110132	+	525	Protein_125	transposase	-
C1P61_RS24680	110215..110487	+	273	WP_000019161.1	hypothetical protein	-
C1P61_RS24685	110468..111610	+	1143	Protein_127	IS91 family transposase	-
C1P61_RS24690	111743..112477	+	735	Protein_128	IS21-like element IS100kyp family helper ATPase IstB	-
C1P61_RS24695	112891..113193	+	303	WP_162147618.1	DUF1778 domain-containing protein	Antitoxin
C1P61_RS24700	113184..113666	+	483	WP_000405248.1	GNAT family N-acetyltransferase	Toxin
C1P61_RS24705	113734..114257	+	524	Protein_131	transposase	-
C1P61_RS26710	114300..114983	-	684	Protein_132	IS3 family transposase	-
C1P61_RS26715	114966..115763	-	798	Protein_133	T3SS effector E3 ubiquitin-protein ligase IpaH1.4	-
C1P61_RS24715	115838..116110	+	273	WP_000019161.1	hypothetical protein	-
C1P61_RS24720	116091..117299	+	1209	WP_000198558.1	IS91 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	ospE2 / ipaH1.4 / ipaH7.8 / ipaH4.5 / ipgB2 / ospD1 / ospF / ospD2 / ospC2 / ospC4 / ospB / icsP/sopA / nleE / icsA/virG / virA / spa40 / spa40 / spa29 / spa9 / spa24 / spa33 / spa32 / spa13 / spa47 / spa15 / mxiA / mxiC / mxiD / mxiE / mxiM / mxiL / mxiN / mxiK / mxiJ / mxiI / mxiH / mxiG / ipgF / ipgE / ipgD / icsB / ipgA / ipgB1 / ipgC / ipaB / ipaC / ipaD / ipaA / ipaJ / ospC3 / ospG / ipaH9.8 / ospI / ospC2 / ospD3/senA / ospC1	1..182697	182697
	-	inside	IScluster/Tn	-	ospC3	101458..120889	19431

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (29-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (19-94)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17736.50 Da
• Isoelectric Point: 7.0382

>T95799 WP_000405248.1 NZ_CP026775:113184-113666 [Shigella dysenteriae]
MEINVTAPALLTDEHILQPFDCGNEVLSNWLRGRAMKNQMLNASRTFVICLEDTLRVVGYYSLATGSVTHAELGRSLRHN
MPNPVPVVLLGRLAVDVCTQGHGFGKWLLSDAIHRVVNLADQVGIKAVMVHAIDDDARAFYERFGFVQSVVAPNTLFYKV

Nucleotide

• Length: 483 bp

>T95799 NZ_CP026775:113184-113666 [Shigella dysenteriae]
ATGGAAATAAATGTCACCGCGCCAGCATTGTTGACGGATGAGCATATACTTCAGCCATTTGACTGTGGAAATGAGGTGCT
AAGTAACTGGTTACGTGGTCGGGCTATGAAAAACCAGATGCTCAATGCTTCTCGCACGTTCGTTATTTGCTTGGAGGATA
CTTTACGTGTTGTGGGATACTACTCATTAGCTACTGGTTCCGTCACTCATGCCGAGCTCGGTCGCAGTTTGCGGCATAAC
ATGCCCAATCCTGTCCCTGTTGTTCTGTTAGGGCGTCTGGCTGTTGATGTTTGTACCCAGGGGCATGGTTTCGGTAAATG
GCTACTAAGTGATGCCATTCACCGGGTTGTCAATCTGGCTGATCAGGTTGGCATTAAGGCTGTTATGGTACATGCAATTG
ATGATGACGCCAGAGCATTTTATGAGCGTTTTGGTTTTGTTCAGTCAGTTGTGGCACCTAATACCTTGTTTTATAAAGTC
TGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11737.32 Da
• Isoelectric Point: 4.9167

>AT95799 WP_162147618.1 NZ_CP026775:112891-113193 [Shigella dysenteriae]
MEFFMSTAASVRKTPREHQINIRATDEERAVIDYAASLVNKNRTDFIMELAYQEAKNIILDQRLFVLDNERYDSFITQLE
APVQNAEGRERLMAVKPEWK

Nucleotide

• Length: 303 bp

>AT95799 NZ_CP026775:112891-113193 [Shigella dysenteriae]
ATGGAGTTTTTTATGTCTACAGCTGCAAGCGTCCGTAAAACACCGCGAGAACATCAGATTAACATCCGGGCAACTGATGA
GGAACGAGCCGTTATTGATTATGCTGCGAGCTTGGTCAATAAGAACCGTACCGATTTCATCATGGAACTGGCGTACCAGG
AAGCTAAAAACATTATCCTGGATCAACGTTTATTTGTGCTGGATAACGAACGTTATGATTCGTTTATTACGCAACTGGAA
GCTCCTGTGCAGAATGCAGAAGGGCGTGAACGTCTGATGGCGGTAAAGCCTGAATGGAAATAA

Structures

Toxin

• AlphaFold DB: A0A090NWA7