Detailed information of TA system TA095777

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 31827..32602
• Replicon: plasmid unnamed3
• Accession: NZ_CP026771
• Organism: Shigella flexneri Y strain 93-3063

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: Q7BEG9
• Locus tag: C1P87_RS24510
• Protein ID: WP_000405245.1
• Coordinates: 31827..32309 (-)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: Q7BEG8
• Locus tag: C1P87_RS24515
• Protein ID: WP_010921625.1
• Coordinates: 32300..32602 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C1P87_RS24495	28241..29932	+	1692	WP_134796984.1	T3SS effector E3 ubiquitin-protein ligase IpaH2.5	-
C1P87_RS24500	29984..31193	+	1210	Protein_29	IS3 family transposase	-
C1P87_RS24505	31236..31759	-	524	Protein_30	IS3-like element ISSfl11 family transposase	-
C1P87_RS24510	31827..32309	-	483	WP_000405245.1	GNAT family N-acetyltransferase	Toxin
C1P87_RS24515	32300..32602	-	303	WP_010921625.1	DUF1778 domain-containing protein	Antitoxin
C1P87_RS24525	32874..33146	+	273	WP_000019158.1	hypothetical protein	-
C1P87_RS24530	33127..34334	+	1208	WP_134798318.1	IS91 family transposase	-
C1P87_RS25890	34506..34634	+	129	Protein_35	IS5/IS1182 family transposase	-
C1P87_RS24545	34783..36237	-	1455	WP_075332747.1	type 3 secretion system effector OspC2	-
C1P87_RS27140	36554..36733	-	180	WP_014526559.1	hypothetical protein	-
C1P87_RS24550	36647..37260	+	614	Protein_38	IS1 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	icsB / ipgA / ipgB1 / ipgC / ipaB / ipaC / ipaD / ipaA / ipaJ / ospC3 / ipaH2.5 / ospC2 / ipaH7.8 / ipaH4.5 / ospD3/senA / ospC1 / ospE2 / ipgB2 / ospD1 / ospF / ospD2 / ospC2 / ospC4 / ospB / icsP/sopA / nleE / ospE1 / ipaH1.4 / ospG / ipaH9.8 / ospI / papC / icsA/virG / virA / spa40 / spa29 / spa9 / spa24 / spa33 / spa32 / spa13 / spa47 / spa15 / mxiA / mxiC / mxiD / mxiE / mxiM / mxiL / mxiN / mxiK / mxiJ / mxiI / mxiH / mxiG / ipgF / ipgE / ipgD	1..220759	220759
	-	inside	IScluster/Tn	-	ospC3 / ipaH2.5 / ospC2	12316..39619	27303

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (29-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (19-94)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17750.52 Da
• Isoelectric Point: 7.0382

>T95777 WP_000405245.1 NZ_CP026771:c32309-31827 [Shigella flexneri Y]
MEINVTAPALLTDEHILQPFDCGNEVLSNWLRGRAMKNQMLNASRTFVICLEDTLRIVGYYSLATGSVTHAELGRSLRHN
MPNPVPVVLLGRLAVDVCTQGHGFGKWLLSDAIHRVVNLADQVGIKAVMVHAIDDDARAFYERFGFVQSVVAPNTLFYKV

Nucleotide

• Length: 483 bp

>T95777 NZ_CP026771:c32309-31827 [Shigella flexneri Y]
ATGGAAATAAATGTCACCGCGCCAGCATTGTTGACGGATGAGCATATACTTCAGCCATTTGACTGTGGAAATGAGGTGCT
AAGTAACTGGTTACGTGGTCGGGCTATGAAAAACCAGATGCTCAATGCTTCTCGCACGTTCGTTATTTGCTTGGAGGATA
CTTTACGTATTGTGGGATACTACTCATTAGCTACTGGTTCCGTCACTCATGCCGAGCTTGGTCGCAGTTTGCGGCATAAC
ATGCCCAATCCTGTCCCTGTTGTTCTGTTAGGGCGTCTGGCTGTTGATGTTTGTACCCAGGGGCATGGTTTCGGTAAATG
GCTACTAAGTGATGCCATTCACCGGGTTGTCAATCTGGCTGATCAGGTTGGCATTAAGGCTGTTATGGTACATGCAATTG
ATGATGACGCCAGAGCATTTTATGAGCGTTTTGGTTTTGTTCAGTCAGTTGTGGCACCTAATACCTTGTTTTATAAAGTC
TGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11689.28 Da
• Isoelectric Point: 4.9167

>AT95777 WP_010921625.1 NZ_CP026771:c32602-32300 [Shigella flexneri Y]
MEVFMSTAASVRKTPREHQINIRATDEERAVIDYAASLVNKNRTDFIMELAYQEAKNIILDQRLFVLDNERYDSFITQLE
APVQNAEGRERLMAVKPEWK

Nucleotide

• Length: 303 bp

>AT95777 NZ_CP026771:c32602-32300 [Shigella flexneri Y]
ATGGAGGTTTTTATGTCTACAGCTGCAAGCGTCCGTAAAACACCGCGAGAACATCAGATTAACATCCGGGCAACTGATGA
GGAACGAGCCGTTATTGATTATGCTGCGAGCTTGGTCAATAAGAACCGTACCGATTTCATCATGGAACTGGCGTACCAGG
AAGCTAAAAACATTATCCTGGATCAACGTTTATTTGTGCTGGATAACGAACGTTATGATTCGTTTATTACGCAACTGGAA
GCTCCTGTGCAGAATGCAGAAGGGCGTGAACGTCTGATGGCGGTAAAGCCTGAATGGAAATAA

Structures

Toxin

• AlphaFold DB: Q7BEG9

Antitoxin

• AlphaFold DB: Q7BEG8