Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/ElaA-DUF1778
Location 24245..25002 Replicon plasmid unnamed3
Accession NZ_CP026771
Organism Shigella flexneri Y strain 93-3063

Toxin (Protein)


Gene name tacT Uniprot ID D2AJG5
Locus tag C1P87_RS24475 Protein ID WP_000501974.1
Coordinates 24517..25002 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID Q31SN8
Locus tag C1P87_RS24470 Protein ID WP_011114751.1
Coordinates 24245..24529 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C1P87_RS24435 20306..20980 + 675 WP_001088287.1 IS66-like element accessory protein TnpA -
C1P87_RS24440 20977..21327 + 351 WP_005048975.1 IS66 family insertion sequence element accessory protein TnpB -
C1P87_RS24445 21324..22925 + 1602 WP_134798250.1 IS66-like element ISSfl3 family transposase -
C1P87_RS26405 23179..23343 - 165 WP_001346193.1 hypothetical protein -
C1P87_RS24470 24245..24529 + 285 WP_011114751.1 DUF1778 domain-containing protein Antitoxin
C1P87_RS24475 24517..25002 + 486 WP_000501974.1 GNAT family N-acetyltransferase Toxin
C1P87_RS24480 25175..25456 - 282 Protein_25 IS4/IS5 family transposase -
C1P87_RS24485 25523..26679 + 1157 Protein_26 IS3 family transposase -
C1P87_RS24490 26794..28022 + 1229 WP_094081542.1 IS3-like element IS2 family transposase -
C1P87_RS24495 28241..29932 + 1692 WP_134796984.1 T3SS effector E3 ubiquitin-protein ligase IpaH2.5 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - icsB / ipgA / ipgB1 / ipgC / ipaB / ipaC / ipaD / ipaA / ipaJ / ospC3 / ipaH2.5 / ospC2 / ipaH7.8 / ipaH4.5 / ospD3/senA / ospC1 / ospE2 / ipgB2 / ospD1 / ospF / ospD2 / ospC2 / ospC4 / ospB / icsP/sopA / nleE / ospE1 / ipaH1.4 / ospG / ipaH9.8 / ospI / papC / icsA/virG / virA / spa40 / spa29 / spa9 / spa24 / spa33 / spa32 / spa13 / spa47 / spa15 / mxiA / mxiC / mxiD / mxiE / mxiM / mxiL / mxiN / mxiK / mxiJ / mxiI / mxiH / mxiG / ipgF / ipgE / ipgD 1..220759 220759
- inside IScluster/Tn - ospC3 / ipaH2.5 / ospC2 12316..39619 27303


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(27-151)

Antitoxin

Vibrio phage ICP1, Orf50

(12-88)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17814.63 Da        Isoelectric Point: 9.8822

>T95776 WP_000501974.1 NZ_CP026771:24517-25002 [Shigella flexneri Y]
MGCVTAPEPLSSFHQVAEFVSSEAVLDDWLKQKELKNQAIGATRTFVVCRKGTQQIVGFYSLATGSVNHTEATGNLRRNM
PDPIPVIILARLAVDVSFRGKGLGADLLHDAVRRCYRVAENIGVRAIMVHALTENAKQFYIHHGFKPSKTQVQTLFLKLP
Q

Download         Length: 486 bp

>T95776 NZ_CP026771:24517-25002 [Shigella flexneri Y]
ATGGGATGTGTAACTGCACCAGAACCTTTATCCTCATTTCATCAGGTCGCTGAATTTGTCAGCAGTGAAGCTGTACTTGA
TGACTGGTTAAAGCAGAAGGAGCTAAAAAATCAGGCAATCGGAGCCACCCGGACTTTTGTTGTATGCAGAAAAGGTACGC
AACAAATAGTCGGTTTTTACTCACTGGCCACTGGTAGTGTCAATCATACAGAAGCTACAGGTAACCTTCGGCGCAATATG
CCAGACCCCATTCCTGTAATTATACTTGCACGCCTGGCTGTAGATGTGTCATTTCGAGGAAAAGGGCTTGGCGCGGATTT
ACTTCATGATGCAGTTCGTCGTTGTTACAGGGTTGCCGAAAATATCGGTGTGCGTGCAATTATGGTTCATGCGCTTACGG
AAAACGCAAAACAGTTTTATATTCATCATGGTTTTAAGCCATCAAAAACTCAGGTGCAAACATTGTTTCTTAAACTCCCT
CAATAG

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10871.52 Da        Isoelectric Point: 4.6812

>AT95776 WP_011114751.1 NZ_CP026771:24245-24529 [Shigella flexneri Y]
MIYGGFMKSGVQLNLRARESQRILIDAAAEILHKSRTDFILEMACKAAEDVILDRRVFNFNDRQYEEFIEMLDAPVADDP
AIEKLLARKPQWDV

Download         Length: 285 bp

>AT95776 NZ_CP026771:24245-24529 [Shigella flexneri Y]
ATGATATACGGAGGCTTTATGAAATCAGGTGTTCAGCTCAATCTCAGAGCAAGAGAATCACAGCGGATACTCATTGATGC
TGCTGCAGAAATTCTTCATAAGTCGCGTACAGATTTCATTCTGGAAATGGCCTGTAAAGCTGCTGAAGATGTTATTCTCG
ACCGGCGGGTGTTTAATTTTAATGACAGGCAATATGAAGAGTTCATTGAAATGCTTGATGCTCCGGTTGCAGATGATCCT
GCCATCGAAAAATTGCTGGCAAGGAAACCTCAATGGGATGTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A822PN28

Antitoxin

Source ID Structure
AlphaFold DB A0A4V1CUJ1

References