Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 22373..22965 Replicon plasmid unnamed1
Accession NZ_CP026769
Organism Shigella flexneri Y strain 93-3063

Toxin (Protein)


Gene name hicA Uniprot ID V0AGQ2
Locus tag C1P87_RS23825 Protein ID WP_000520549.1
Coordinates 22717..22965 (-) Length 83 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID V0AWG0
Locus tag C1P87_RS23820 Protein ID WP_000681613.1
Coordinates 22373..22720 (-) Length 116 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C1P87_RS23785 18375..18701 - 327 WP_000008644.1 TrbC/VirB2 family protein -
C1P87_RS23790 18715..19335 - 621 WP_000157549.1 lytic transglycosylase domain-containing protein -
C1P87_RS23795 19692..19907 - 216 WP_000760375.1 EexN family lipoprotein -
C1P87_RS23800 19904..20197 - 294 WP_000134733.1 sigma factor-like helix-turn-helix DNA-binding protein -
C1P87_RS23805 20274..21503 - 1230 WP_016231858.1 DNA relaxase/nickase TaxC -
C1P87_RS23810 21507..22010 - 504 WP_000781814.1 relaxosome-coupling protein TaxA -
C1P87_RS23820 22373..22720 - 348 WP_000681613.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
C1P87_RS23825 22717..22965 - 249 WP_000520549.1 type II toxin-antitoxin system HicA family toxin Toxin
C1P87_RS23830 23275..23568 - 294 WP_001401669.1 hypothetical protein -
C1P87_RS23835 23583..23957 - 375 WP_000844303.1 tail-associated lysozyme -
C1P87_RS23840 24009..24227 - 219 WP_001407769.1 hypothetical protein -
C1P87_RS23850 24762..24989 - 228 WP_000801440.1 hypothetical protein -
C1P87_RS23855 24979..25227 - 249 WP_001230706.1 hypothetical protein -
C1P87_RS23860 25224..25766 - 543 WP_001083906.1 hypothetical protein -
C1P87_RS23865 25871..26386 - 516 WP_001025395.1 J domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..27054 27054


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(20-81)

Antitoxin

Uncharacterised protein family HicB

(54-104)


Sequences

Toxin        


Download         Length: 83 a.a.        Molecular weight: 9422.08 Da        Isoelectric Point: 10.4800

>T95775 WP_000520549.1 NZ_CP026769:c22965-22717 [Shigella flexneri Y]
MGKTDKLLAKFLNSKKTFEWDELVVLFSSLGYVKKEMQGSRVRFFNAEINHTILMHRPHPESYIKGGTLKAIKQNLKEAG
LL

Download         Length: 249 bp

>T95775 NZ_CP026769:c22965-22717 [Shigella flexneri Y]
ATGGGGAAAACAGATAAGCTACTGGCAAAGTTTTTAAACAGTAAAAAAACGTTTGAATGGGATGAACTGGTCGTTTTGTT
TTCCTCTTTGGGATATGTCAAAAAGGAAATGCAGGGGTCAAGAGTGCGGTTTTTCAATGCTGAAATCAATCACACCATAT
TAATGCATCGTCCACATCCGGAAAGTTATATCAAAGGTGGTACGCTGAAAGCAATTAAACAGAATCTGAAAGAGGCTGGA
TTATTATGA

Antitoxin


Download         Length: 116 a.a.        Molecular weight: 13113.84 Da        Isoelectric Point: 4.6723

>AT95775 WP_000681613.1 NZ_CP026769:c22720-22373 [Shigella flexneri Y]
MKHLKYKGYLGTVEPDFENNVLYGKLAFIRDLVTYEASTLAELEQEFKTSVDLYLQSCVEDGKEPDTPFKGVFNVRLDPE
LHRRVAEMAMEEDLSLNAFVNKALEKEVSNHRAGA

Download         Length: 348 bp

>AT95775 NZ_CP026769:c22720-22373 [Shigella flexneri Y]
ATGAAACATTTAAAATATAAAGGATATTTAGGCACGGTTGAACCCGATTTTGAAAATAATGTCCTGTATGGAAAACTGGC
GTTTATTCGGGACCTGGTAACTTATGAGGCTTCTACATTAGCTGAACTGGAACAGGAGTTTAAAACATCTGTTGATCTGT
ATTTACAGTCTTGTGTGGAGGATGGAAAGGAACCTGATACCCCGTTTAAAGGCGTGTTTAACGTCAGGCTTGATCCAGAA
CTGCATCGTCGGGTAGCAGAAATGGCGATGGAAGAAGATTTATCGTTGAATGCCTTTGTTAATAAGGCTCTGGAAAAAGA
AGTCAGCAATCATCGCGCAGGGGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CIX3

Antitoxin

Source ID Structure
AlphaFold DB A0A829CLC1

References