Detailed information of TA system TA095754

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 78341..78966
• Replicon: plasmid unnamed
• Accession: NZ_CP026767
• Organism: Shigella boydii strain 59-248

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: C1P58_RS25010
• Protein ID: WP_073690939.1
• Coordinates: 78341..78739 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: Q31SG2
• Locus tag: C1P58_RS25015
• Protein ID: WP_000450530.1
• Coordinates: 78739..78966 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C1P58_RS24985	74315..75265	+	951	WP_012449328.1	virulence factor VirK	-
C1P58_RS24990	75330..76274	+	945	WP_005027201.1	lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase	-
C1P58_RS24995	76428..77099	-	672	Protein_85	IS630 family transposase	-
C1P58_RS25000	77115..77812	-	698	Protein_86	IS1 family transposase	-
C1P58_RS25005	77868..78332	+	465	Protein_87	conjugal transfer protein TraD	-
C1P58_RS25010	78341..78739	-	399	WP_073690939.1	tRNA(fMet)-specific endonuclease VapC	Toxin
C1P58_RS25015	78739..78966	-	228	WP_000450530.1	toxin-antitoxin system antitoxin VapB	Antitoxin
C1P58_RS25020	79051..79467	+	417	WP_171548060.1	conjugative relaxase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	ospI / ospI / ospC3 / ipaJ / ipaA / ipaD / ipaC / ipaB / ipgC / ipgB1 / ipgA / icsB / ipgD / ipgE / ipgF / mxiG / mxiH / mxiI / mxiJ / mxiK / mxiN / mxiL / mxiM / mxiE / mxiD / mxiC / mxiA / spa15 / spa47 / spa13 / spa32 / spa33 / spa24 / spa9 / spa29 / spa40 / virA / icsA/virG / papC / ipaH9.8 / ospC2 / nleE / icsP/sopA / ospB / ospC3 / ospC4 / ospD2 / ospF / ospD1 / ipgB2 / ipaH1.4 / ipaH4.5 / ipaH7.8 / ospD3/senA / ospC1 / senB / ospE2 / ospI / ospC3 / ipaJ / ipaA / ipaA / ipaA / ipaD	1..209166	209166
	-	inside	IScluster/Tn	-	ipaH9.8	63036..77618	14582

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-51)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14846.07 Da
• Isoelectric Point: 7.8605

>T95754 WP_073690939.1 NZ_CP026767:c78739-78341 [Shigella boydii]
MLKFMLDTNICIFTIKNKPASVRERFNLNQGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIIVTNDTREFERVGGLRTEDWS

Nucleotide

• Length: 399 bp

>T95754 NZ_CP026767:c78739-78341 [Shigella boydii]
ATGCTGAAGTTTATGCTCGATACCAACATCTGCATTTTTACGATAAAGAACAAACCCGCCAGCGTCAGGGAGCGTTTTAA
CCTGAACCAGGGGAGAATGTGTATCAGTTCGGTCACCCTGATGGAGCTGATATATGGTGCAGAAAAAAGCCAGATGCCTG
AACGTAATCTCGCTGTGATCGAGGGATTTGTTTCCCGCATTGATGTTCTGGATTACGACGCTGCTGCAGCCACACACACC
GGCCAGATAAGAGCAGAACTTGCCCGTCAGGGACGCCCTGTCGGGCCATTTGATCAAATGATCGCAGGTCATGCCCGCAG
TCGGGGGCTGATTATTGTGACTAATGACACCCGGGAATTTGAACGTGTGGGCGGCCTGAGAACTGAAGACTGGAGCTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8535.59 Da
• Isoelectric Point: 4.7513

>AT95754 WP_000450530.1 NZ_CP026767:c78966-78739 [Shigella boydii]
METTVFLSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAGETWDEWFDGHSVSADFMDNREQPGMQERESF

Nucleotide

• Length: 228 bp

>AT95754 NZ_CP026767:c78966-78739 [Shigella boydii]
ATGGAAACCACCGTATTTCTCAGCAACCGCAGCCAGGCGGTCAGACTGCCAAAAGCGGTTGCATTGCCGGAAAACGTAAA
ACGCGTTGAAGTGATTGCTGTCGGACGAACCAGAATCATTACGCCAGCCGGAGAGACATGGGACGAATGGTTCGACGGAC
ACAGCGTCAGCGCCGATTTTATGGATAACAGGGAACAGCCCGGCATGCAGGAAAGGGAGTCATTCTGA

Structures

Antitoxin

• AlphaFold DB: Q31SG2