Detailed information of TA system TA095753

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 66461..66986
• Replicon: plasmid unnamed
• Accession: NZ_CP026767
• Organism: Shigella boydii strain 59-248

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: -
• Locus tag: C1P58_RS24935
• Protein ID: WP_073691655.1
• Coordinates: 66681..66986 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: Q7BEK0
• Locus tag: C1P58_RS24930
• Protein ID: WP_000813626.1
• Coordinates: 66461..66679 (+)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C1P58_RS26325	62599..63154	+	556	Protein_68	fimbria/pilus outer membrane usher protein	-
C1P58_RS26330	64344..64553	-	210	WP_073691819.1	hypothetical protein	-
C1P58_RS24920	64608..65776	+	1169	WP_134802526.1	IS3 family transposase	-
C1P58_RS24930	66461..66679	+	219	WP_000813626.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
C1P58_RS24935	66681..66986	+	306	WP_073691655.1	type II toxin-antitoxin system toxin CcdB	Toxin
C1P58_RS24940	67010..67117	+	108	WP_023592908.1	transposase domain-containing protein	-
C1P58_RS24945	67379..69016	+	1638	WP_039076510.1	T3SS effector E3 ubiquitin-protein ligase IpaH9.8	-
C1P58_RS24950	69224..69493	+	270	WP_000079956.1	hypothetical protein	-
C1P58_RS26410	69490..69662	+	173	Protein_77	type II toxin-antitoxin system RelE/ParE family toxin	-
C1P58_RS24960	69726..70423	-	698	Protein_78	IS1 family transposase	-
C1P58_RS24965	70630..70746	+	117	WP_004996477.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	ospI / ospI / ospC3 / ipaJ / ipaA / ipaD / ipaC / ipaB / ipgC / ipgB1 / ipgA / icsB / ipgD / ipgE / ipgF / mxiG / mxiH / mxiI / mxiJ / mxiK / mxiN / mxiL / mxiM / mxiE / mxiD / mxiC / mxiA / spa15 / spa47 / spa13 / spa32 / spa33 / spa24 / spa9 / spa29 / spa40 / virA / icsA/virG / papC / ipaH9.8 / ospC2 / nleE / icsP/sopA / ospB / ospC3 / ospC4 / ospD2 / ospF / ospD1 / ipgB2 / ipaH1.4 / ipaH4.5 / ipaH7.8 / ospD3/senA / ospC1 / senB / ospE2 / ospI / ospC3 / ipaJ / ipaA / ipaA / ipaA / ipaD	1..209166	209166
	-	inside	IScluster/Tn	-	ipaH9.8	63036..77618	14582

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11722.56 Da
• Isoelectric Point: 6.4674

>T95753 WP_073691655.1 NZ_CP026767:66681-66986 [Shigella boydii]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVFVIGEEV
ADLSHRENDIKNAINLMFWGI

Nucleotide

• Length: 306 bp

>T95753 NZ_CP026767:66681-66986 [Shigella boydii]
ATGCAGTTTAAGGTTTACGCCTATAAAAGAGAGAGTCGTTATCGTCTGTTTGTGGATGTACAGAGTGACATTATTGACAC
GCCCGGACGACGGATGGTGATCCCCCTGGCCAGTGCCCGCCTGCTGTCAGATAAAGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACTGATATGGCCAGTGTGCCGGTCTTCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8333.36 Da
• Isoelectric Point: 6.2838

>AT95753 WP_000813626.1 NZ_CP026767:66461-66679 [Shigella boydii]
MKQRITVTIDSDSYQLLKSANVNISGLVNTAMQKEARRLRAERWQAENQQGMAEIARFIEMNGSFADENRDW

Nucleotide

• Length: 219 bp

>AT95753 NZ_CP026767:66461-66679 [Shigella boydii]
ATGAAGCAGCGTATTACTGTCACTATAGACAGTGACAGCTATCAGTTACTTAAATCCGCAAACGTGAATATCTCAGGCCT
GGTAAATACGGCCATGCAAAAAGAGGCCCGCCGTCTGCGTGCGGAACGCTGGCAGGCTGAAAATCAGCAGGGGATGGCTG
AGATCGCCCGTTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA

Structures

Antitoxin

• AlphaFold DB: Q7BEK0