Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09812-MazE
Location 4488199..4488774 Replicon chromosome
Accession NZ_CP026766
Organism Shigella boydii strain 59-248

Toxin (Protein)


Gene name pemK Uniprot ID I6EL31
Locus tag C1P58_RS23405 Protein ID WP_000373726.1
Coordinates 4488457..4488774 (+) Length 106 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID I6EL32
Locus tag C1P58_RS23400 Protein ID WP_000288814.1
Coordinates 4488199..4488456 (+) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C1P58_RS23365 4483406..4484433 + 1028 Protein_4422 IS3 family transposase -
C1P58_RS23370 4484596..4484805 + 210 WP_001044499.1 hypothetical protein -
C1P58_RS23375 4484833..4485198 + 366 WP_000253098.1 hypothetical protein -
C1P58_RS23380 4485211..4486815 + 1605 WP_000628841.1 hypothetical protein -
C1P58_RS23385 4487186..4487647 + 462 WP_000201268.1 hypothetical protein -
C1P58_RS23390 4487644..4487850 + 207 WP_000979502.1 helix-turn-helix domain-containing protein -
C1P58_RS23400 4488199..4488456 + 258 WP_000288814.1 antitoxin Antitoxin
C1P58_RS23405 4488457..4488774 + 318 WP_000373726.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
C1P58_RS23420 4489639..4490523 + 885 WP_000511770.1 integrase domain-containing protein -
C1P58_RS23435 4491235..4492497 - 1263 WP_073691628.1 integrase arm-type DNA-binding domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - ipaH9.8 4369454..4521977 152523


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-105)

Antitoxin

Antitoxin PemI-like

(4-84)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 11293.94 Da        Isoelectric Point: 10.2965

>T95752 WP_000373726.1 NZ_CP026766:4488457-4488774 [Shigella boydii]
MDRGEIWLVSLDPTTGHEQSGKRPVLIVSKASFNTFTRLPVVVPVTSGGNFAREAGFTVSLDGAGTKTTGVIRCDQPRTI
DMGARSGKRLERIPDAVVNEVLARL

Download         Length: 318 bp

>T95752 NZ_CP026766:4488457-4488774 [Shigella boydii]
ATGGACAGAGGAGAAATCTGGCTCGTTTCACTGGATCCCACCACTGGCCATGAACAAAGTGGAAAACGTCCGGTACTCAT
CGTATCGAAGGCATCGTTTAACACGTTCACCCGGTTGCCTGTTGTTGTCCCTGTCACCAGCGGCGGAAACTTTGCCCGCG
AAGCCGGTTTTACCGTTTCACTGGACGGGGCGGGAACAAAAACAACGGGCGTTATTCGCTGCGACCAGCCCAGAACCATT
GATATGGGAGCTCGGAGCGGTAAGCGGCTGGAACGTATACCCGATGCTGTCGTGAATGAGGTGCTGGCTCGACTGTAA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9280.76 Da        Isoelectric Point: 4.5821

>AT95752 WP_000288814.1 NZ_CP026766:4488199-4488456 [Shigella boydii]
MYTTRLKKVGGSVMLAVPPAVLKTLGLSTDSEVGMTIDNGCLIIEPQKRPRYSLEELLAQCDPHAEISEEGREWIDAPAV
GKEIL

Download         Length: 258 bp

>AT95752 NZ_CP026766:4488199-4488456 [Shigella boydii]
ATGTATACCACTCGCCTGAAAAAGGTTGGCGGATCCGTCATGCTGGCGGTTCCCCCCGCAGTGCTGAAAACGCTAGGGCT
GTCGACGGACAGCGAAGTGGGCATGACCATTGATAATGGCTGCCTGATTATTGAACCTCAAAAACGTCCCCGCTATTCGC
TCGAAGAACTGTTGGCGCAGTGCGATCCGCACGCTGAAATAAGCGAAGAGGGTCGGGAATGGATTGATGCGCCTGCGGTG
GGCAAGGAGATCCTGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3T2UX62

Antitoxin

Source ID Structure
AlphaFold DB A0A085HLS8

References