Detailed information of TA system TA095740

Bioinformatically predicted

Overview

TA module

• Type: V
• Classification (family/domain): ghoTS/ghoT-GhoS
• Location: 2934070..2934477
• Replicon: chromosome
• Accession: NZ_CP026766
• Organism: Shigella boydii strain 59-248

Toxin (Protein)

• Gene name: ghoT
• Uniprot ID: L4JJG6
• Locus tag: C1P58_RS15085
• Protein ID: WP_001173343.1
• Coordinates: 2934070..2934243 (-)
• Length: 58 a.a.

Antitoxin (Protein)

• Gene name: ghoS
• Uniprot ID: -
• Locus tag: C1P58_RS15090
• Protein ID: WP_000272166.1
• Coordinates: 2934271..2934477 (-)
• Length: 69 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C1P58_RS15050	2929333..2930526	+	1194	WP_000074489.1	MFS transporter	-
C1P58_RS15065	2931912..2932819	+	908	Protein_2853	IS3 family transposase	-
C1P58_RS15070	2932862..2933227	+	366	Protein_2854	transposase	-
C1P58_RS15080	2933241..2933951	+	711	Protein_2855	lysine--tRNA ligase	-
C1P58_RS15085	2934070..2934243	-	174	WP_001173343.1	type V toxin-antitoxin system toxin GhoT	Toxin
C1P58_RS15090	2934271..2934477	-	207	WP_000272166.1	type V toxin-antitoxin system endoribonuclease antitoxin GhoS	Antitoxin
C1P58_RS15095	2934495..2935192	-	698	Protein_2858	IS1 family transposase	-
C1P58_RS15100	2935249..2935344	-	96	Protein_2859	type V toxin-antitoxin system endoribonuclease antitoxin GhoS	-
C1P58_RS15105	2935571..2935843	-	273	WP_000405647.1	N-acetyltransferase	-
C1P58_RS15110	2935855..2936085	-	231	WP_000371700.1	(4Fe-4S)-binding protein	-
C1P58_RS15115	2936266..2937897	+	1632	WP_001216454.1	sensor histidine kinase	-
C1P58_RS15120	2937894..2938613	+	720	WP_073691944.1	two-component system response regulator DcuR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	IScluster/Tn	-	-	2930671..2934998	4327

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin GhoT/OrtT (4-57)

Antitoxin

• Endoribonuclease antitoxin GhoS (1-64)

Sequences

Toxin

Protein

• Length: 58 a.a.
• Molecular weight: 6555.06 Da
• Isoelectric Point: 10.2288

>T95740 WP_001173343.1 NZ_CP026766:c2934243-2934070 [Shigella boydii]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Nucleotide

• Length: 174 bp

>T95740 NZ_CP026766:c2934243-2934070 [Shigella boydii]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 69 a.a.
• Molecular weight: 7915.95 Da
• Isoelectric Point: 3.8086

>AT95740 WP_000272166.1 NZ_CP026766:c2934477-2934271 [Shigella boydii]
MYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDIDFDLNIMTVDDYFRQIYK

Nucleotide

• Length: 207 bp

>AT95740 NZ_CP026766:c2934477-2934271 [Shigella boydii]
ATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATTCCTTCGCAAT
AACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATTGATTTTGACC
TCAACATTATGACAGTTGATGACTATTTCCGTCAGATTTACAAGTAG

Structures

Toxin

• AlphaFold DB: A0A7U9QI41