Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 3862659..3863290 Replicon chromosome
Accession NZ_CP026765
Organism Shigella flexneri strain 04-3145

Toxin (Protein)


Gene name hicA Uniprot ID A0A822PMR8
Locus tag C1P85_RS20370 Protein ID WP_001259384.1
Coordinates 3863015..3863290 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag C1P85_RS20365 Protein ID WP_000593555.1
Coordinates 3862659..3863018 (-) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C1P85_RS20340 (3858791) 3858791..3859735 + 945 WP_000947076.1 nickel ABC transporter permease subunit NikB -
C1P85_RS20345 (3859732) 3859732..3860565 + 834 WP_001008974.1 nickel ABC transporter permease subunit NikC -
C1P85_RS20350 (3860565) 3860565..3861329 + 765 WP_001136248.1 nickel import ATP-binding protein NikD -
C1P85_RS20355 (3861326) 3861326..3862132 + 807 WP_000173660.1 nickel import ATP-binding protein NikE -
C1P85_RS20360 (3862138) 3862138..3862539 + 402 WP_001190069.1 nickel-responsive transcriptional regulator NikR -
C1P85_RS20365 (3862659) 3862659..3863018 - 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
C1P85_RS20370 (3863015) 3863015..3863290 - 276 WP_001259384.1 type II toxin-antitoxin system HicA family toxin Toxin
C1P85_RS20375 (3863349) 3863349..3864473 - 1125 WP_001216257.1 ABC transporter permease -
C1P85_RS20380 (3864473) 3864473..3867208 - 2736 WP_000149173.1 ribosome-associated ATPase/putative transporter RbbA -
C1P85_RS20385 (3867205) 3867205..3868272 - 1068 WP_000361470.1 HlyD family secretion protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-81)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10146.89 Da        Isoelectric Point: 10.3748

>T95733 WP_001259384.1 NZ_CP026765:c3863290-3863015 [Shigella flexneri]
MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
CDWLLSIGVKP

Download         Length: 276 bp

>T95733 NZ_CP026765:c3863290-3863015 [Shigella flexneri]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGCAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
TGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT95733 WP_000593555.1 NZ_CP026765:c3863018-3862659 [Shigella flexneri]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT95733 NZ_CP026765:c3863018-3862659 [Shigella flexneri]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCAGCTGTTATTACCTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGTATTCAGGGGCTGCAAAAAGAAGGAG
AGCTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A822PMR8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References