Detailed information of TA system TA095720

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 1887974..1888500
• Replicon: chromosome
• Accession: NZ_CP026765
• Organism: Shigella flexneri strain 04-3145

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1EXL1
• Locus tag: C1P85_RS10135
• Protein ID: WP_000323025.1
• Coordinates: 1888213..1888500 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: S1F6D3
• Locus tag: C1P85_RS10130
• Protein ID: WP_000534858.1
• Coordinates: 1887974..1888213 (+)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C1P85_RS10110 (1885569)	1885569..1885997	-	429	Protein_1885	fimbrial protein	-
C1P85_RS10115 (1886054)	1886054..1886751	+	698	WP_227804319.1	IS1 family transposase	-
C1P85_RS10120 (1886769)	1886769..1887647	+	879	Protein_1887	IS3-like element IS600 family transposase	-
C1P85_RS10125 (1887644)	1887644..1887949	-	306	WP_071818640.1	hypothetical protein	-
C1P85_RS10130 (1887974)	1887974..1888213	+	240	WP_000534858.1	type II toxin-antitoxin system antitoxin RelB	Antitoxin
C1P85_RS10135 (1888213)	1888213..1888500	+	288	WP_000323025.1	type II toxin-antitoxin system mRNA interferase RelE	Toxin
C1P85_RS10140 (1888572)	1888572..1888727	+	156	WP_000813254.1	type I toxin-antitoxin system toxin HokD	-
C1P85_RS10145 (1888945)	1888945..1889196	+	252	WP_000980987.1	protein Rem	-
C1P85_RS10150 (1889263)	1889263..1889541	+	279	Protein_1893	hypothetical protein	-
C1P85_RS10155 (1889543)	1889543..1890592	+	1050	WP_001265249.1	DUF968 domain-containing protein	-
C1P85_RS10160 (1890605)	1890605..1890961	+	357	WP_000904111.1	RusA family crossover junction endodeoxyribonuclease	-
C1P85_RS10165 (1890976)	1890976..1891797	+	822	WP_000762884.1	antitermination protein	-
C1P85_RS10185 (1892718)	1892718..1892826	+	109	Protein_1897	DUF3927 family protein	-
C1P85_RS10190 (1893107)	1893107..1893442	-	336	WP_000871291.1	anti-adapter protein IraM	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1877844..1899291	21447

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-86)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-79)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11225.22 Da
• Isoelectric Point: 10.1967

>T95720 WP_000323025.1 NZ_CP026765:1888213-1888500 [Shigella flexneri]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Nucleotide

• Length: 288 bp

>T95720 NZ_CP026765:1888213-1888500 [Shigella flexneri]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 9071.48 Da
• Isoelectric Point: 4.5230

>AT95720 WP_000534858.1 NZ_CP026765:1887974-1888213 [Shigella flexneri]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Nucleotide

• Length: 240 bp

>AT95720 NZ_CP026765:1887974-1888213 [Shigella flexneri]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Structures

Toxin

• PDB: 4V7K
• PDB: 4V7J
• PDB: 4FXI
• PDB: 4FXE
• PDB: 2KC8
• PDB: 4FXH
• PDB: 2KC9

Antitoxin

• PDB: 4FXE
• PDB: 2KC8
• PDB: 2K29
• AlphaFold DB: A0A4V1CTS8