Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 138635..139410 Replicon plasmid unnamed
Accession NZ_CP026763
Organism Shigella boydii strain NCTC 9850

Toxin (Protein)


Gene name tacT Uniprot ID Q326V1
Locus tag C1P50_RS25150 Protein ID WP_000405248.1
Coordinates 138635..139117 (-) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID Q7BEG8
Locus tag C1P50_RS25155 Protein ID WP_010921625.1
Coordinates 139108..139410 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C1P50_RS25120 134138..135085 - 948 WP_005005342.1 omptin family outer membrane protease IcsP -
C1P50_RS25395 136047..136184 + 138 WP_012421760.1 hypothetical protein -
C1P50_RS25130 136466..136618 - 153 Protein_164 IS3 family transposase -
C1P50_RS25135 136674..137366 + 693 WP_000625263.1 type III secretion system effector cysteine methyltransferase OspZ -
C1P50_RS25140 137390..138466 - 1077 Protein_166 IS3 family transposase -
C1P50_RS25150 138635..139117 - 483 WP_000405248.1 GNAT family N-acetyltransferase Toxin
C1P50_RS25155 139108..139410 - 303 WP_010921625.1 DUF1778 domain-containing protein Antitoxin
C1P50_RS25165 139675..139947 + 273 WP_000019158.1 hypothetical protein -
C1P50_RS25170 139928..141136 + 1209 WP_134795483.1 IS91 family transposase -
C1P50_RS25320 141308..141436 + 129 Protein_171 IS5/IS1182 family transposase -
C1P50_RS25185 141585..143039 - 1455 WP_000701110.1 type 3 secretion system effector OspC2 -
C1P50_RS25190 143464..144308 + 845 WP_094096542.1 IS5 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - ospG / ipaH9.8 / ospC3 / ipaH4.5 / ipaH7.8 / senB / ospC1 / ospD3/senA / ospE2 / ipaH1.4 / ospI / ipgB2 / ospD1 / ospF / ospD2 / ospC4 / ospB / icsP/sopA / nleE / ospC2 1..150497 150497
- inside IScluster/Tn - ipgB2 / ospD1 / ospF / ospD2 / ospC4 / ospB / icsP/sopA / nleE / ospC2 104979..146648 41669


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(29-149)

Antitoxin

Vibrio phage ICP1, Orf50

(19-94)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17736.50 Da        Isoelectric Point: 7.0382

>T95713 WP_000405248.1 NZ_CP026763:c139117-138635 [Shigella boydii]
MEINVTAPALLTDEHILQPFDCGNEVLSNWLRGRAMKNQMLNASRTFVICLEDTLRVVGYYSLATGSVTHAELGRSLRHN
MPNPVPVVLLGRLAVDVCTQGHGFGKWLLSDAIHRVVNLADQVGIKAVMVHAIDDDARAFYERFGFVQSVVAPNTLFYKV

Download         Length: 483 bp

>T95713 NZ_CP026763:c139117-138635 [Shigella boydii]
ATGGAAATAAATGTCACCGCGCCAGCATTGTTGACGGATGAGCATATACTTCAGCCATTTGACTGTGGAAATGAGGTGCT
AAGTAACTGGTTACGTGGTCGGGCTATGAAAAACCAGATGCTCAATGCTTCTCGCACGTTCGTTATTTGCTTGGAGGATA
CTTTACGTGTTGTGGGATACTACTCATTAGCTACTGGTTCCGTCACTCATGCCGAGCTTGGTCGCAGTTTGCGGCATAAC
ATGCCCAATCCTGTCCCTGTTGTTCTGTTAGGGCGTCTGGCTGTTGATGTTTGTACCCAGGGGCATGGTTTCGGTAAATG
GCTACTAAGTGATGCCATTCACCGGGTTGTCAATCTGGCTGATCAGGTTGGCATTAAGGCTGTTATGGTACATGCAATTG
ATGATGACGCCAGAGCATTTTATGAGCGTTTTGGTTTTGTTCAGTCAGTTGTGGCACCTAATACCTTGTTTTATAAAGTC
TGA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11689.28 Da        Isoelectric Point: 4.9167

>AT95713 WP_010921625.1 NZ_CP026763:c139410-139108 [Shigella boydii]
MEVFMSTAASVRKTPREHQINIRATDEERAVIDYAASLVNKNRTDFIMELAYQEAKNIILDQRLFVLDNERYDSFITQLE
APVQNAEGRERLMAVKPEWK

Download         Length: 303 bp

>AT95713 NZ_CP026763:c139410-139108 [Shigella boydii]
ATGGAGGTTTTTATGTCTACAGCTGCAAGCGTCCGTAAAACACCGCGAGAACATCAGATTAACATCCGGGCAACTGATGA
GGAACGAGCCGTTATTGATTATGCTGCGAGCTTGGTCAATAAGAACCGTACCGATTTCATCATGGAACTGGCGTACCAGG
AAGCTAAAAACATTATCTTGGATCAACGTTTATTTGTGCTGGATAACGAACGTTATGATTCGTTTATTACGCAACTGGAA
GCTCCTGTGCAGAATGCAGAAGGGCGTGAACGTCTGATGGCGGTAAAGCCTGAATGGAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090NWA7

Antitoxin

Source ID Structure
AlphaFold DB Q7BEG8

References