Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 21853..22378 Replicon plasmid unnamed
Accession NZ_CP026763
Organism Shigella boydii strain NCTC 9850

Toxin (Protein)


Gene name ccdB Uniprot ID Q326Z8
Locus tag C1P50_RS24310 Protein ID WP_001159860.1
Coordinates 21853..22158 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID Q7BEK0
Locus tag C1P50_RS24315 Protein ID WP_000813626.1
Coordinates 22160..22378 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C1P50_RS24275 17593..17709 - 117 WP_004996477.1 hypothetical protein -
C1P50_RS24280 17916..18613 + 698 Protein_18 IS1 family transposase -
C1P50_RS24285 18628..19113 - 486 WP_000705603.1 hypothetical protein -
C1P50_RS25670 19142..19368 - 227 Protein_20 type II toxin-antitoxin system RelE/ParE family toxin -
C1P50_RS24295 19365..19634 - 270 WP_000079956.1 hypothetical protein -
C1P50_RS24300 19842..21479 - 1638 WP_134795474.1 T3SS effector E3 ubiquitin-protein ligase IpaH9.8 -
C1P50_RS24305 21722..21817 - 96 Protein_23 transposase domain-containing protein -
C1P50_RS24310 21853..22158 - 306 WP_001159860.1 type II toxin-antitoxin system toxin CcdB Toxin
C1P50_RS24315 22160..22378 - 219 WP_000813626.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
C1P50_RS24325 22971..23243 + 273 WP_012421738.1 hypothetical protein -
C1P50_RS25675 24584..25027 - 444 WP_201797260.1 hypothetical protein -
C1P50_RS24340 25310..25525 - 216 Protein_29 DDE-type integrase/transposase/recombinase -
C1P50_RS24350 25888..26637 + 750 WP_000828661.1 phosphatase PAP2 family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - ospG / ipaH9.8 / ospC3 / ipaH4.5 / ipaH7.8 / senB / ospC1 / ospD3/senA / ospE2 / ipaH1.4 / ospI / ipgB2 / ospD1 / ospF / ospD2 / ospC4 / ospB / icsP/sopA / nleE / ospC2 1..150497 150497
- inside IScluster/Tn - ospG / ipaH9.8 / ospC3 / ipaH4.5 / ipaH7.8 11987..55336 43349


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11736.59 Da        Isoelectric Point: 6.4674

>T95711 WP_001159860.1 NZ_CP026763:c22158-21853 [Shigella boydii]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPIFVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T95711 NZ_CP026763:c22158-21853 [Shigella boydii]
ATGCAGTTTAAGGTTTACGCCTATAAAAGAGAGAGTCGTTATCGTTTGTTTGTGGATGTACAGAGTGACATTATTGACAC
GCCCGGACGACGGATGGTGATCCCCCTGGCCAGTGCCCGCCTGCTGTCAGATAAAGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACTGATATGGCCAGTGTGCCGATCTTCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8333.36 Da        Isoelectric Point: 6.2838

>AT95711 WP_000813626.1 NZ_CP026763:c22378-22160 [Shigella boydii]
MKQRITVTIDSDSYQLLKSANVNISGLVNTAMQKEARRLRAERWQAENQQGMAEIARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT95711 NZ_CP026763:c22378-22160 [Shigella boydii]
ATGAAGCAGCGTATTACTGTCACTATAGACAGTGACAGCTATCAGTTACTTAAATCCGCAAACGTGAATATCTCAGGCCT
GGTAAATACGGCCATGCAAAAAGAGGCCCGCCGTCTGCGTGCGGAACGCTGGCAGGCTGAAAATCAGCAGGGGATGGCTG
AGATCGCCCGTTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TTN3

Antitoxin

Source ID Structure
AlphaFold DB Q7BEK0

References