Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 8723..9348 Replicon plasmid unnamed
Accession NZ_CP026763
Organism Shigella boydii strain NCTC 9850

Toxin (Protein)


Gene name vapC Uniprot ID A0A2S8DLI0
Locus tag C1P50_RS24235 Protein ID WP_000911319.1
Coordinates 8950..9348 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID Q31SG2
Locus tag C1P50_RS24230 Protein ID WP_000450530.1
Coordinates 8723..8950 (+) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C1P50_RS24225 8226..8519 - 294 WP_171548087.1 relaxase domain-containing protein -
C1P50_RS24230 8723..8950 + 228 WP_000450530.1 toxin-antitoxin system antitoxin VapB Antitoxin
C1P50_RS24235 8950..9348 + 399 WP_000911319.1 tRNA(fMet)-specific endonuclease VapC Toxin
C1P50_RS24240 9357..11545 - 2189 Protein_10 type IV conjugative transfer system coupling protein TraD -
C1P50_RS24245 11793..12490 + 698 Protein_11 IS1 family transposase -
C1P50_RS24250 12506..13177 + 672 Protein_12 IS630 family transposase -
C1P50_RS24255 13332..14276 - 945 WP_012421740.1 lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - ospG / ipaH9.8 / ospC3 / ipaH4.5 / ipaH7.8 / senB / ospC1 / ospD3/senA / ospE2 / ipaH1.4 / ospI / ipgB2 / ospD1 / ospF / ospD2 / ospC4 / ospB / icsP/sopA / nleE / ospC2 1..150497 150497
- inside IScluster/Tn - ospG / ipaH9.8 / ospC3 / ipaH4.5 / ipaH7.8 11987..55336 43349


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-125)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14887.17 Da        Isoelectric Point: 8.5264

>T95710 WP_000911319.1 NZ_CP026763:8950-9348 [Shigella boydii]
MLKFMLDTNICIFTIKNKPASVRERFNLNQGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHT
VQIRAELARQGRPVGPFDQMIAGHARSRGLIIVTNNTREFERVGGLRTEDWS

Download         Length: 399 bp

>T95710 NZ_CP026763:8950-9348 [Shigella boydii]
ATGCTGAAGTTTATGCTCGATACCAACATCTGCATTTTTACGATAAAGAACAAACCCGCCAGCGTCAGGGAGCGTTTTAA
CCTGAACCAGGGGAGAATGTGTATCAGTTCGGTCACCCTGATGGAGCTGATATATGGTGCAGAAAAAAGCCAGATGCCTG
AACGTAATCTCGCTGTGATCGAGGGATTTGTTTCCCGCATTGATGTTCTGGATTACGACGCTGCTGCAGCCACACACACC
GTCCAGATAAGAGCAGAACTTGCCCGTCAGGGACGCCCTGTCGGGCCATTTGATCAAATGATCGCAGGTCATGCCCGCAG
TCGGGGGCTGATTATTGTGACTAATAACACCCGGGAATTTGAACGTGTGGGCGGCCTGAGAACTGAAGACTGGAGCTGA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8535.59 Da        Isoelectric Point: 4.7513

>AT95710 WP_000450530.1 NZ_CP026763:8723-8950 [Shigella boydii]
METTVFLSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAGETWDEWFDGHSVSADFMDNREQPGMQERESF

Download         Length: 228 bp

>AT95710 NZ_CP026763:8723-8950 [Shigella boydii]
ATGGAAACCACCGTATTTCTCAGCAACCGCAGCCAGGCGGTCAGACTGCCAAAAGCGGTTGCATTGCCGGAAAACGTAAA
ACGCGTTGAAGTGATTGCTGTCGGACGAACCAGAATCATTACGCCAGCCGGAGAGACATGGGACGAATGGTTCGACGGAC
ACAGCGTCAGCGCCGATTTTATGGATAACAGGGAACAGCCCGGCATGCAGGAAAGGGAGTCATTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2S8DLI0

Antitoxin

Source ID Structure
AlphaFold DB Q31SG2

References