Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 2516131..2516714 Replicon chromosome
Accession NZ_CP026762
Organism Shigella boydii strain NCTC 9850

Toxin (Protein)


Gene name mazF Uniprot ID Q31XK8
Locus tag C1P50_RS13515 Protein ID WP_000254733.1
Coordinates 2516379..2516714 (+) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S1P3W5
Locus tag C1P50_RS13510 Protein ID WP_000581937.1
Coordinates 2516131..2516379 (+) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C1P50_RS13500 2512470..2513771 + 1302 WP_000046829.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -
C1P50_RS13505 2513819..2516053 + 2235 WP_039059511.1 GTP diphosphokinase -
C1P50_RS13510 2516131..2516379 + 249 WP_000581937.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
C1P50_RS13515 2516379..2516714 + 336 WP_000254733.1 endoribonuclease MazF Toxin
C1P50_RS13520 2516785..2517576 + 792 WP_001071648.1 nucleoside triphosphate pyrophosphohydrolase -
C1P50_RS13525 2517804..2519441 + 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
C1P50_RS13530 2519529..2520827 + 1299 WP_000036723.1 phosphopyruvate hydratase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12167.15 Da        Isoelectric Point: 8.7218

>T95707 WP_000254733.1 NZ_CP026762:2516379-2516714 [Shigella boydii]
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKR
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

>T95707 NZ_CP026762:2516379-2516714 [Shigella boydii]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGA
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9355.74 Da        Isoelectric Point: 4.4665

>AT95707 WP_000581937.1 NZ_CP026762:2516131-2516379 [Shigella boydii]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp

>AT95707 NZ_CP026762:2516131-2516379 [Shigella boydii]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB Q31XK8

Antitoxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CQY
PDB 1MVF
PDB 2MRN
PDB 2MRU
AlphaFold DB A0A7U9LMB4

References