Detailed information of TA system TA095695

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 681374..682205
• Replicon: chromosome
• Accession: NZ_CP026762
• Organism: Shigella boydii strain NCTC 9850

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: S1P968
• Locus tag: C1P50_RS03830
• Protein ID: WP_000854814.1
• Coordinates: 681831..682205 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: B3Y195
• Locus tag: C1P50_RS03825
• Protein ID: WP_001285585.1
• Coordinates: 681374..681742 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C1P50_RS03795	677521..677820	-	300	Protein_693	IS1 family transposase	-
C1P50_RS03805	679133..679963	+	831	WP_039059216.1	DUF945 domain-containing protein	-
C1P50_RS03810	680045..680524	+	480	WP_000860059.1	antirestriction protein	-
C1P50_RS03815	680540..681016	+	477	WP_012421636.1	RadC family protein	-
C1P50_RS03820	681079..681300	+	222	WP_000692323.1	DUF987 domain-containing protein	-
C1P50_RS03825	681374..681742	+	369	WP_001285585.1	type IV toxin-antitoxin system toxin CbtA	Antitoxin
C1P50_RS03830	681831..682205	+	375	WP_000854814.1	type IV toxin-antitoxin system toxin CbtA	Toxin
C1P50_RS03835	682202..682396	+	195	WP_000988600.1	hypothetical protein	-
C1P50_RS25450	682409..682522	+	114	WP_001161660.1	DUF957 domain-containing protein	-
C1P50_RS03845	682911..684014	+	1104	WP_134795469.1	IS110-like element ISSso6 family transposase	-
C1P50_RS03855	685557..685796	-	240	Protein_705	nickel/cobalt efflux protein RcnA	-
C1P50_RS03860	685917..686189	+	273	WP_000019944.1	Ni(II)/Co(II)-binding transcriptional repressor RcnR	-
C1P50_RS03865	686412..687200	+	789	WP_001195636.1	hydroxyethylthiazole kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	IScluster/Tn	-	-	666440..685157	18717

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13898.94 Da
• Isoelectric Point: 7.8522

>T95695 WP_000854814.1 NZ_CP026762:681831-682205 [Shigella boydii]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Nucleotide

• Length: 375 bp

>T95695 NZ_CP026762:681831-682205 [Shigella boydii]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13593.48 Da
• Isoelectric Point: 6.3139

>AT95695 WP_001285585.1 NZ_CP026762:681374-681742 [Shigella boydii]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCGYVYLAVYPTPEMKN

Nucleotide

• Length: 369 bp

>AT95695 NZ_CP026762:681374-681742 [Shigella boydii]
GTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCAAAGCCGATACCCTCAGCAGTTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Structures

Toxin

• AlphaFold DB: A0A829FY50

Antitoxin

• AlphaFold DB: A0A7U9LW60