Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 12919..13472 Replicon plasmid tig00000059_pilon
Accession NZ_CP026759
Organism Klebsiella aerogenes strain AR_0062

Toxin (Protein)


Gene name ccdB Uniprot ID A0A7X5QH88
Locus tag AM441_RS26615 Protein ID WP_029403621.1
Coordinates 13158..13472 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID A0A7X5QHA0
Locus tag AM441_RS26610 Protein ID WP_029403619.1
Coordinates 12919..13155 (+) Length 79 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM441_RS26580 8582..9226 + 645 WP_104891174.1 type IV secretion system protein -
AM441_RS26585 9238..10152 + 915 WP_029403614.1 P-type conjugative transfer protein TrbG -
AM441_RS26590 10156..10602 + 447 WP_109949323.1 hypothetical protein -
AM441_RS26595 10595..11809 + 1215 WP_164720183.1 TrbI/VirB10 family protein -
AM441_RS26600 11832..12314 + 483 WP_071434325.1 single-stranded DNA-binding protein -
AM441_RS26605 12404..12751 + 348 WP_071434319.1 hypothetical protein -
AM441_RS26610 12919..13155 + 237 WP_029403619.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
AM441_RS26615 13158..13472 + 315 WP_029403621.1 CcdB family protein Toxin
AM441_RS26625 13859..14695 + 837 WP_104891177.1 replication initiation protein -
AM441_RS27330 15587..15763 - 177 WP_164720184.1 hypothetical protein -
AM441_RS26630 15792..16103 - 312 WP_164720185.1 CcgAII protein -
AM441_RS26635 16256..16534 - 279 WP_070133932.1 plasmid stability/partitioning protein -
AM441_RS26640 16610..17272 - 663 WP_036944392.1 plasmid stability/partitioning protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..25674 25674


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-103)

Antitoxin

Post-segregation antitoxin CcdA

(9-78)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11602.53 Da        Isoelectric Point: 5.7681

>T95690 WP_029403621.1 NZ_CP026759:13158-13472 [Klebsiella aerogenes]
MQFTVYSNTGKSAVYPLLIDVTSDIIGQLNRRIVIPLLPVGMYPNGMRPERLTPLIRLVDDNEYVVMTHEMASIPARSLG
AAFCDASSYRKQIKDAIDFLLDGI

Download         Length: 315 bp

>T95690 NZ_CP026759:13158-13472 [Klebsiella aerogenes]
ATGCAATTCACTGTATATAGTAATACAGGGAAAAGTGCCGTTTATCCCCTGCTGATTGATGTAACAAGCGATATTATCGG
CCAGTTAAACCGGAGGATTGTTATCCCCCTGTTACCTGTTGGAATGTACCCTAATGGTATGCGACCGGAGCGCCTGACTC
CGCTTATCAGGCTGGTTGATGATAATGAGTATGTAGTCATGACTCATGAAATGGCGAGTATTCCTGCTCGTTCTTTGGGG
GCTGCGTTCTGTGATGCTTCTTCTTATCGTAAGCAGATCAAGGATGCTATTGATTTTCTTCTCGATGGTATTTAA

Antitoxin


Download         Length: 79 a.a.        Molecular weight: 9020.12 Da        Isoelectric Point: 5.6870

>AT95690 WP_029403619.1 NZ_CP026759:12919-13155 [Klebsiella aerogenes]
MAVTQRPMQTVTMTVKRTLLTRAREAGINLSATLTAALDAELRQYEAKRWQEENSEALEALNRFHDEHGCFSDEYRTF

Download         Length: 237 bp

>AT95690 NZ_CP026759:12919-13155 [Klebsiella aerogenes]
ATGGCTGTAACACAACGACCAATGCAAACCGTAACCATGACGGTAAAACGAACCTTGCTCACCCGTGCGCGTGAGGCTGG
AATTAATTTGAGCGCCACCCTGACCGCTGCCCTCGATGCTGAATTACGTCAGTACGAAGCCAAGAGGTGGCAAGAGGAAA
ACAGCGAAGCACTGGAAGCCCTCAATCGCTTCCACGACGAACACGGCTGTTTCAGTGATGAATACAGGACATTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7X5QH88

Antitoxin

Source ID Structure
AlphaFold DB A0A7X5QHA0

References