Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 4806913..4807463 Replicon chromosome
Accession NZ_CP026755
Organism Escherichia coli strain AR_0077

Toxin (Protein)


Gene name ccdB Uniprot ID A0A0H3MLP2
Locus tag AM456_RS25180 Protein ID WP_001160965.1
Coordinates 4807149..4807463 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1NMQ0
Locus tag AM456_RS25175 Protein ID WP_000125568.1
Coordinates 4806913..4807146 (+) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM456_RS25150 4801987..4802274 + 288 WP_000203741.1 ferredoxin-like protein FixX -
AM456_RS25155 4802332..4803663 + 1332 WP_001183201.1 MFS transporter -
AM456_RS25160 4803771..4804301 + 531 WP_000600732.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
AM456_RS25165 4804294..4806156 + 1863 WP_000377117.1 glutathione-regulated potassium-efflux system protein KefC -
AM456_RS25170 4806348..4806827 + 480 WP_000624375.1 type 3 dihydrofolate reductase -
AM456_RS25175 4806913..4807146 + 234 WP_000125568.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
AM456_RS25180 4807149..4807463 + 315 WP_001160965.1 CcdB family protein Toxin
AM456_RS25185 4807460..4808308 - 849 WP_000257195.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
AM456_RS25190 4808315..4808692 - 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
AM456_RS25195 4808695..4809516 - 822 WP_001065381.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
AM456_RS25200 4809513..4810502 - 990 WP_000241247.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
AM456_RS25205 4810502..4811788 - 1287 WP_000800457.1 peptidylprolyl isomerase SurA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-103)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11725.75 Da        Isoelectric Point: 9.0851

>T95676 WP_001160965.1 NZ_CP026755:4807149-4807463 [Escherichia coli]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLIDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Download         Length: 315 bp

>T95676 NZ_CP026755:4807149-4807463 [Escherichia coli]
ATGCAATTTACGGTATACCGCAGTCGCGGCAGGAACGCCGCGTTTCCCTTTGTTATCGATGTTACCAGCGACATTATTGG
TGAGATTAATCGCCGTATCGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAATC
CAATCCTTTTACTGATAGATGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAAGGAGCATTAGATTTTATGCTCGACGGGATTTAA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8560.55 Da        Isoelectric Point: 5.8062

>AT95676 WP_000125568.1 NZ_CP026755:4806913-4807146 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENTEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT95676 NZ_CP026755:4806913-4807146 [Escherichia coli]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCCGCAACACGTTGGCGTGAAGAGAACA
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3MLP2

Antitoxin

Source ID Structure
AlphaFold DB A0A829GEF2

References