Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4686459..4687035 Replicon chromosome
Accession NZ_CP026755
Organism Escherichia coli strain AR_0077

Toxin (Protein)


Gene name shpA Uniprot ID A0A2U2VE01
Locus tag AM456_RS24570 Protein ID WP_001131965.1
Coordinates 4686459..4686746 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A2U2VE39
Locus tag AM456_RS24575 Protein ID WP_000063158.1
Coordinates 4686733..4687035 (+) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM456_RS24545 4681868..4683280 - 1413 WP_094030254.1 PLP-dependent aminotransferase family protein -
AM456_RS24550 4683457..4683621 + 165 WP_094030253.1 DUF1127 domain-containing protein -
AM456_RS24555 4683663..4684001 - 339 WP_094030252.1 endoribonuclease SymE -
AM456_RS24560 4684216..4684884 - 669 Protein_4390 DUF3387 domain-containing protein -
AM456_RS26240 4684878..4685051 - 174 Protein_4391 SAM-dependent DNA methyltransferase -
AM456_RS24565 4685096..4686262 - 1167 WP_000800823.1 restriction endonuclease -
AM456_RS24570 4686459..4686746 + 288 WP_001131965.1 BrnT family toxin Toxin
AM456_RS24575 4686733..4687035 + 303 WP_000063158.1 BrnA antitoxin family protein Antitoxin
AM456_RS24580 4687069..4688025 - 957 WP_053292465.1 GTPase -
AM456_RS24585 4688036..4688239 - 204 WP_000467859.1 YbdD/YjiX family protein -
AM456_RS24590 4688289..4690439 - 2151 WP_001314407.1 pyruvate/proton symporter BtsT -
AM456_RS24600 4690707..4691927 - 1221 WP_001561360.1 amino acid deaminase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11223.71 Da        Isoelectric Point: 7.4021

>T95675 WP_001131965.1 NZ_CP026755:4686459-4686746 [Escherichia coli]
MPMEFEWDANKAKSNRVKHGIRFEDAVLVFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T95675 NZ_CP026755:4686459-4686746 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGCAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTAGTGTTTGATGATCCACAACATTTGTCACAGCAAGAACGCATAGAAAACGGTGAGTATCGCTGGCAGACAATCGGCC
TGGTTTACGGCATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAATCGCTATGAGCATGGTTAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11537.99 Da        Isoelectric Point: 9.4646

>AT95675 WP_000063158.1 NZ_CP026755:4686733-4687035 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDSDVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT95675 NZ_CP026755:4686733-4687035 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCGCTGGCGAA
TAAATCCGACGAAGATATTGATTACAGTGATATCCCACCTTCCAGCGATGAGCAATGGTCTAACGCAGAACGCGGTAAGT
TTTATCGGCCATTGAAAACTCAGGCCTCGGTGCGTATTGATTCTGATGTGATGGAATGGTTGAAGCGACCAGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2U2VE01

Antitoxin

Source ID Structure
AlphaFold DB A0A2U2VE39

References