Detailed information of TA system TA095674

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 4561873..4562395
• Replicon: chromosome
• Accession: NZ_CP026755
• Organism: Escherichia coli strain AR_0077

Toxin (Protein)

• Gene name: relE
• Uniprot ID: V0SDB6
• Locus tag: AM456_RS24005
• Protein ID: WP_001105432.1
• Coordinates: 4562105..4562395 (+)
• Length: 97 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: AM456_RS24000
• Protein ID: WP_032172221.1
• Coordinates: 4561873..4562115 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM456_RS23975	4557658..4559031	+	1374	WP_021563928.1	UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl- meso-diaminopimelate ligase	-
AM456_RS23980	4559114..4559665	-	552	WP_000166270.1	ribosome-associated protein	-
AM456_RS23985	4559759..4561111	+	1353	WP_016242696.1	metalloprotease PmbA	-
AM456_RS23990	4561295..4561681	+	387	WP_021576676.1	cytochrome b562	-
AM456_RS24000	4561873..4562115	+	243	WP_032172221.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
AM456_RS24005	4562105..4562395	+	291	WP_001105432.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AM456_RS24010	4562396..4562860	-	465	WP_032172223.1	anaerobic ribonucleoside-triphosphate reductase-activating protein	-
AM456_RS24020	4563040..4565178	-	2139	WP_000187799.1	anaerobic ribonucleoside-triphosphate reductase	-
AM456_RS24025	4565572..4567227	-	1656	WP_089582899.1	alpha,alpha-phosphotrehalase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-82)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-67)

Sequences

Toxin

Protein

• Length: 97 a.a.
• Molecular weight: 11311.21 Da
• Isoelectric Point: 10.0238

>T95674 WP_001105432.1 NZ_CP026755:4562105-4562395 [Escherichia coli]
MNYELAFDPRALKEWQKLGVTVREQFKKKLEDVLKRPRNPSAKLRDLPDCYKIKLRTLGYRLVYSVNDKELLVLVIAIGK
RENSAVYEDATKRLDE

Nucleotide

• Length: 291 bp

>T95674 NZ_CP026755:4562105-4562395 [Escherichia coli]
ATGAACTATGAGCTGGCGTTTGATCCGCGGGCGCTGAAAGAGTGGCAAAAACTGGGCGTGACAGTCCGGGAACAATTTAA
AAAAAAGCTTGAGGATGTGCTGAAAAGGCCGCGCAATCCATCGGCTAAACTGCGGGATTTACCGGACTGCTACAAAATTA
AGCTGCGCACACTGGGATATCGCCTGGTCTATTCCGTCAATGATAAAGAGTTACTCGTGTTGGTGATAGCTATTGGTAAG
AGAGAGAACTCAGCCGTTTATGAAGACGCGACAAAACGGCTGGATGAGTGA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9141.56 Da
• Isoelectric Point: 5.1369

>AT95674 WP_032172221.1 NZ_CP026755:4561873-4562115 [Escherichia coli]
MATLNVRLDDKLKNEAYAVLEKLNITPTEAVRLLFQYVAENGRMPVKTLTVSDSEEALLRTVRERLSNPQEAIKVRLDEL

Nucleotide

• Length: 243 bp

>AT95674 NZ_CP026755:4561873-4562115 [Escherichia coli]
ATGGCCACACTTAACGTCCGCCTGGATGACAAACTGAAAAATGAGGCTTATGCGGTGCTGGAAAAATTAAATATTACGCC
TACTGAAGCAGTGCGGCTGCTGTTTCAGTACGTTGCGGAAAATGGACGAATGCCGGTAAAAACGCTCACTGTCAGTGATA
GTGAAGAAGCCTTGTTACGCACTGTCAGGGAACGACTTTCCAACCCACAAGAGGCCATTAAGGTCCGACTGGATGAACTA
TGA

Structures

Toxin

• AlphaFold DB: V0SDB6