Detailed information of TA system TA095673

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): chpSB/PRK09812-ChpS
• Location: 4550328..4550923
• Replicon: chromosome
• Accession: NZ_CP026755
• Organism: Escherichia coli strain AR_0077

Toxin (Protein)

• Gene name: chpB
• Uniprot ID: V0SXT4
• Locus tag: AM456_RS23940
• Protein ID: WP_000239581.1
• Coordinates: 4550573..4550923 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: chpS
• Uniprot ID: L4JJX7
• Locus tag: AM456_RS23935
• Protein ID: WP_001223213.1
• Coordinates: 4550328..4550579 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM456_RS23925	4545993..4549772	+	3780	WP_094030088.1	autotransporter assembly complex protein TamB	-
AM456_RS23930	4549775..4550116	+	342	WP_001219160.1	gamma-glutamylcyclotransferase	-
AM456_RS23935	4550328..4550579	+	252	WP_001223213.1	type II toxin-antitoxin system antitoxin ChpS	Antitoxin
AM456_RS23940	4550573..4550923	+	351	WP_000239581.1	endoribonuclease toxin ChpB	Toxin
AM456_RS23945	4551002..4551532	-	531	WP_000055072.1	inorganic diphosphatase	-
AM456_RS23950	4551842..4552798	+	957	WP_000265933.1	galactofuranose ABC transporter substrate-binding protein YtfQ	-
AM456_RS23955	4552931..4554433	+	1503	WP_001600915.1	sugar ABC transporter ATP-binding protein	-
AM456_RS23960	4554447..4555469	+	1023	WP_001298067.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (8-114)

Antitoxin

• SpoVT-AbrB domain (6-49)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12453.41 Da
• Isoelectric Point: 6.2206

>T95673 WP_000239581.1 NZ_CP026755:4550573-4550923 [Escherichia coli]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAAGEVVEEALLRLQAVVE

Nucleotide

• Length: 351 bp

>T95673 NZ_CP026755:4550573-4550923 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGGTGAGGTGGTGGAAGAGGC
GTTATTACGTTTGCAGGCGGTGGTGGAATAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9331.64 Da
• Isoelectric Point: 4.6509

>AT95673 WP_001223213.1 NZ_CP026755:4550328-4550579 [Escherichia coli]
MRITIKRWGNSSGMVIPNVVMKELNLRPGQSVEAQVSNNQLILTPISRRYSLDELLAQCDMNATELSEQDVWGKSTPAGD
EIW

Nucleotide

• Length: 252 bp

>AT95673 NZ_CP026755:4550328-4550579 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTTCAGGTATGGTCATTCCTAATGTTGTGATGAAAGAACTTAACTTACG
GCCGGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCACGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Structures

Toxin

• AlphaFold DB: V0SXT4

Antitoxin

• AlphaFold DB: L4JJX7