Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 3656712..3657343 Replicon chromosome
Accession NZ_CP026755
Organism Escherichia coli strain AR_0077

Toxin (Protein)


Gene name hicA Uniprot ID D8A4M2
Locus tag AM456_RS19595 Protein ID WP_001259770.1
Coordinates 3657068..3657343 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag AM456_RS19590 Protein ID WP_000593555.1
Coordinates 3656712..3657071 (-) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM456_RS19565 3652844..3653788 + 945 WP_000947080.1 nickel ABC transporter permease subunit NikB -
AM456_RS19570 3653785..3654618 + 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
AM456_RS19575 3654618..3655382 + 765 WP_094030326.1 nickel import ATP-binding protein NikD -
AM456_RS19580 3655379..3656185 + 807 WP_000173687.1 nickel import ATP-binding protein NikE -
AM456_RS19585 3656191..3656592 + 402 WP_001189000.1 nickel-responsive transcriptional regulator NikR -
AM456_RS19590 3656712..3657071 - 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
AM456_RS19595 3657068..3657343 - 276 WP_001259770.1 type II toxin-antitoxin system HicA family toxin Toxin
AM456_RS19600 3657416..3658540 - 1125 WP_001314210.1 ABC transporter permease -
AM456_RS19605 3658540..3661275 - 2736 WP_094030325.1 ribosome-associated ATPase/putative transporter RbbA -
AM456_RS19610 3661272..3662339 - 1068 WP_094030324.1 HlyD family efflux transporter periplasmic adaptor subunit -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 3656712..3666178 9466


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10170.90 Da        Isoelectric Point: 10.6128

>T95671 WP_001259770.1 NZ_CP026755:c3657343-3657068 [Escherichia coli]
MRTLVQALRKKQKNTLDQIFKSPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T95671 NZ_CP026755:c3657343-3657068 [Escherichia coli]
ATGAGAACCCTGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAATCTCCTGTTCCTCAAGG
GATTAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGTAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT95671 WP_000593555.1 NZ_CP026755:c3657071-3656712 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT95671 NZ_CP026755:c3657071-3656712 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AGCTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACATTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTAAATACTTA
TATTATTGAAACGCTAAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FQ35

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References