Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 976272..977056 Replicon chromosome
Accession NZ_CP026755
Organism Escherichia coli strain AR_0077

Toxin (Protein)


Gene name TacT2 Uniprot ID V0T0H9
Locus tag AM456_RS05795 Protein ID WP_000613626.1
Coordinates 976272..976766 (-) Length 165 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID B1LI61
Locus tag AM456_RS05800 Protein ID WP_001110446.1
Coordinates 976763..977056 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM456_RS05775 971465..972562 + 1098 WP_000589300.1 flagellar basal body P-ring protein FlgI -
AM456_RS05780 972562..973503 + 942 WP_012311956.1 flagellar assembly peptidoglycan hydrolase FlgJ -
AM456_RS05785 973569..975212 + 1644 WP_094029801.1 flagellar hook-associated protein FlgK -
AM456_RS05790 975224..976177 + 954 WP_001212763.1 flagellar hook-associated protein FlgL -
AM456_RS05795 976272..976766 - 495 WP_000613626.1 GNAT family N-acetyltransferase Toxin
AM456_RS05800 976763..977056 - 294 WP_001110446.1 DUF1778 domain-containing protein Antitoxin
AM456_RS05805 977190..980375 - 3186 WP_077516769.1 ribonuclease E -
AM456_RS05815 980948..981907 + 960 WP_000846342.1 23S rRNA pseudouridine(955/2504/2580) synthase RluC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(81-144)

Antitoxin

Vibrio phage ICP1, Orf50

(14-88)


Sequences

Toxin        


Download         Length: 165 a.a.        Molecular weight: 18084.00 Da        Isoelectric Point: 8.2617

>T95649 WP_000613626.1 NZ_CP026755:c976766-976272 [Escherichia coli]
MIQAPEPLMARHQFTSFCSGVETMDNWLKQRALKNQLAGASRTFVSCDTYSNVLAYYSLASSAVETYVATGRFRRNMPEP
IPVVVLGRLAIDKSLQGQGIGRAMVRDAGLRVLQAAEVIGIRGMLVHALSDQAREFYLRVGFEPSPVDSMILMATLADLQ
ECLK

Download         Length: 495 bp

>T95649 NZ_CP026755:c976766-976272 [Escherichia coli]
ATGATTCAGGCCCCCGAGCCACTCATGGCAAGGCATCAATTCACCTCTTTTTGCTCGGGAGTAGAAACGATGGATAACTG
GCTAAAGCAGAGAGCCCTAAAAAATCAGCTTGCCGGCGCATCACGTACATTTGTTAGCTGTGATACGTATAGCAACGTTT
TGGCATATTATTCGCTTGCTTCCAGCGCCGTTGAAACGTATGTCGCGACAGGACGATTTCGCCGAAATATGCCAGAACCG
ATCCCTGTTGTAGTACTTGGTCGTCTGGCAATAGATAAATCATTACAGGGTCAGGGCATCGGTCGTGCGATGGTCAGAGA
TGCCGGTCTACGAGTACTACAGGCGGCTGAAGTCATTGGTATTCGCGGAATGTTGGTCCATGCGTTATCAGACCAGGCCC
GTGAGTTTTATCTGCGAGTGGGGTTTGAACCATCGCCTGTCGATTCTATGATACTGATGGCAACATTGGCTGATTTGCAG
GAATGTCTTAAATAG

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11079.67 Da        Isoelectric Point: 10.5423

>AT95649 WP_001110446.1 NZ_CP026755:c977056-976763 [Escherichia coli]
MPAANNIPARRESLNLRIKPAERDLIDRAAKARGKNRTEFVLEAARAAAEEALIEQRIFRIDSDDYQLFLNRLDQPPAPN
AALRKTMQTPAPWEQKK

Download         Length: 294 bp

>AT95649 NZ_CP026755:c977056-976763 [Escherichia coli]
ATGCCAGCAGCCAATAACATTCCAGCCAGGCGCGAATCTTTAAACTTGCGCATAAAACCCGCAGAACGCGATCTTATTGA
CCGGGCCGCTAAAGCCCGAGGAAAAAATCGCACCGAATTTGTCCTTGAGGCCGCCAGAGCCGCAGCTGAAGAGGCGCTCA
TTGAGCAGAGAATTTTCAGGATCGATTCTGATGATTACCAGCTATTTTTGAATCGTCTGGATCAGCCCCCCGCGCCCAAT
GCTGCATTACGCAAAACCATGCAAACACCCGCGCCGTGGGAGCAAAAAAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0T0H9

Antitoxin

Source ID Structure
AlphaFold DB A0A829G775

References