Detailed information of TA system TA095648

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-HTH_XRE
• Location: 710468..711173
• Replicon: chromosome
• Accession: NZ_CP026755
• Organism: Escherichia coli strain AR_0077

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1F406
• Locus tag: AM456_RS04405
• Protein ID: WP_000539521.1
• Coordinates: 710787..711173 (-)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: AM456_RS04400
• Protein ID: WP_001280945.1
• Coordinates: 710468..710797 (-)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM456_RS04385	705625..706536	+	912	WP_094030291.1	glutathione ABC transporter permease GsiD	-
AM456_RS04390	706713..709061	+	2349	WP_097465951.1	EAL domain-containing protein	-
AM456_RS04395	709069..710398	+	1330	Protein_687	diguanylate cyclase	-
AM456_RS04400	710468..710797	-	330	WP_001280945.1	DNA-binding transcriptional regulator	Antitoxin
AM456_RS04405	710787..711173	-	387	WP_000539521.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AM456_RS04410	711399..712724	-	1326	WP_000049367.1	30S ribosomal protein S12 methylthiotransferase RimO	-
AM456_RS04415	712937..713320	+	384	WP_000497137.1	biofilm formation regulator BssR	-
AM456_RS04420	713431..714546	+	1116	WP_000555031.1	aldose sugar dehydrogenase YliI	-
AM456_RS04425	714543..715169	-	627	WP_001295292.1	glutathione S-transferase GstB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (1-124)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-94)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14293.45 Da
• Isoelectric Point: 9.9296

>T95648 WP_000539521.1 NZ_CP026755:c711173-710787 [Escherichia coli]
MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVIKKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSKSG
LSSKGTKEIEDDELAAYKKMANAFLAFSNKQIEDLIETGFLIEVKNER

Nucleotide

• Length: 387 bp

>T95648 NZ_CP026755:c711173-710787 [Escherichia coli]
ATGGGTGTGTATAAGGCAAGACGATTCAGCCAAAGCACGAAAAAACTTGGTATTCACGATAAAGTTCTCATGGCTGCCGC
TGAAGAAGTGATGCAGGGCATATGGGAGGCTGATTTAGGTAGCGGTGTCATCAAAAAACGTTTACCCCTCCAACAAGGAA
AGAGTGGTGGAGCCAGAACAATTATCTTTTTTAAATCAGCAAATCATGTGTTTTTTTATGATGGTTGGTCCAAGAGTGGT
CTAAGCAGTAAAGGAACCAAAGAAATCGAAGATGATGAACTGGCGGCCTACAAAAAAATGGCAAATGCATTTCTGGCCTT
TAGCAATAAGCAAATAGAGGATTTGATCGAAACTGGCTTTTTGATTGAGGTGAAAAATGAGCGATAA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11982.08 Da
• Isoelectric Point: 10.0101

>AT95648 WP_001280945.1 NZ_CP026755:c710797-710468 [Escherichia coli]
MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAKVKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSK
WERNEIKPSGPALRILNTLAIKGPEVFAK

Nucleotide

• Length: 330 bp

>AT95648 NZ_CP026755:c710797-710468 [Escherichia coli]
ATGAGCGATAAATTACAAATGATGCTGGAAGATGCAAAGGAACTGAATGCACTGGGCCTGATGTCTGATAGTGAAGTTGA
TGCCATTGCCGTCATGGTCAAAGCTCGTGAAGTCAGCGCCCGTATAGCCAAAGTCAAATCAATGACGGGTGAAGAGATCA
AAGCCATCCGCACCCGATACGGCATGTCACAATCCGTGCTGGCACATACAATGGGAATGTCAAAAGAAAGTGTCTCGAAA
TGGGAACGTAATGAAATAAAACCGAGCGGCCCGGCATTACGTATTTTGAATACATTGGCAATAAAAGGGCCGGAAGTTTT
TGCAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A829GCT6