Detailed information of TA system TA095645

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 262772..263609
• Replicon: chromosome
• Accession: NZ_CP026755
• Organism: Escherichia coli strain AR_0077

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: A0A080JA52
• Locus tag: AM456_RS02005
• Protein ID: WP_032170811.1
• Coordinates: 262772..263314 (-)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: A0A080JFU1
• Locus tag: AM456_RS02010
• Protein ID: WP_001577859.1
• Coordinates: 263298..263609 (-)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM456_RS01985	258312..259223	-	912	WP_000705836.1	2-dehydropantoate 2-reductase	-
AM456_RS01990	259391..259882	+	492	WP_001138904.1	nucleotide binding protein YajQ	-
AM456_RS01995	260010..261374	-	1365	WP_001000978.1	MFS transporter	-
AM456_RS02000	261781..262716	+	936	WP_032171822.1	sel1 repeat family protein	-
AM456_RS02005	262772..263314	-	543	WP_032170811.1	GNAT family N-acetyltransferase	Toxin
AM456_RS02010	263298..263609	-	312	WP_001577859.1	DUF1778 domain-containing protein	Antitoxin
AM456_RS02015	263794..264684	-	891	WP_000971336.1	heme o synthase	-
AM456_RS02020	264696..265025	-	330	WP_000019794.1	cytochrome o ubiquinol oxidase subunit IV	-
AM456_RS02025	265025..265639	-	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
AM456_RS02030	265629..267620	-	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
AM456_RS02035	267642..268589	-	948	WP_001239436.1	cytochrome o ubiquinol oxidase subunit II	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (19-96)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19835.05 Da
• Isoelectric Point: 8.3395

>T95645 WP_032170811.1 NZ_CP026755:c263314-262772 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRTSLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T95645 NZ_CP026755:c263314-262772 [Escherichia coli]
ATGGTCGACAAGCATGAAGAGATTACTCTGCCCATAGTCCTCTCCTGTAATTATCAGTCTGATATTACTTATCCTGGGCA
AAAACAGTTTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCACATCGCTAAAGAAAAGTGTGCGTAATAGCGACT
GTGCGGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATTGGCATATGTACTTTTACGGCATATTCGCTGGAAAAA
CAACGCGTTTCTGGCGTCCTTCAGGGTTCACAGCCTTCAGAGATTGGTGTTGTCAGATTAGTCATGTTGGGGGTGGCGCG
GAAGTATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAATAATTCACCAGGCATTAC
CAATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAACTTTTATGCTCGTCTCGGCTTTGTTCAGCTTTCAGCG
ACACCAAATGCTTTTGGTGCTGTACCGATGTTTTTGGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11400.08 Da
• Isoelectric Point: 10.5548

>AT95645 WP_001577859.1 NZ_CP026755:c263609-263298 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNRLREAAQAVGLDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT95645 NZ_CP026755:c263609-263298 [Escherichia coli]
ATGCCTGGAAAAACAGCCACTCTACCCGATGTCGATAAAACGCTGAAAAATGCGCGGGTTGAACTCAAAACCAGTCCCGA
CGCCAAAAACAGACTTCGTGAAGCTGCGCAAGCCGTTGGGTTAGATTTAAGCGCCTTTATTTTGAGCGCTGCTATGGAAC
GCGCCGAAAGCGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCC
GAACCTGCTCAACCGACGCTCGCACTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Structures

Toxin

• AlphaFold DB: A0A080JA52

Antitoxin

• AlphaFold DB: A0A080JFU1