Detailed information of TA system TA095638

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 42621..43396
• Replicon: plasmid tig00000042_pilon
• Accession: NZ_CP026754
• Organism: Escherichia coli strain AR_0077

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: A0A8D9PPA8
• Locus tag: AM456_RS00335
• Protein ID: WP_000405244.1
• Coordinates: 42621..43103 (-)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: -
• Locus tag: AM456_RS00340
• Protein ID: WP_001346256.1
• Coordinates: 43094..43396 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM456_RS26045	37863..38024	-	162	WP_158660463.1	hypothetical protein	-
AM456_RS00295	38157..39179	+	1023	Protein_49	IS21 family transposase	-
AM456_RS00300	39176..39958	+	783	WP_104888867.1	IS21-like element IS100kyp family helper ATPase IstB	-
AM456_RS00315	40898..41755	-	858	WP_158660464.1	incFII family plasmid replication initiator RepA	-
AM456_RS00320	41748..41822	-	75	WP_001367777.1	RepA leader peptide Tap	-
AM456_RS00330	42054..42311	-	258	WP_000083826.1	replication regulatory protein RepA	-
AM456_RS00335	42621..43103	-	483	WP_000405244.1	GNAT family N-acetyltransferase	Toxin
AM456_RS00340	43094..43396	-	303	WP_001346256.1	DUF1778 domain-containing protein	Antitoxin
AM456_RS00345	43717..43923	-	207	WP_104888871.1	type I toxin-antitoxin system Hok family toxin	-
-	43910..43971	+	62	NuclAT_1	-	-
-	43910..43971	+	62	NuclAT_1	-	-
-	43910..43971	+	62	NuclAT_1	-	-
-	43910..43971	+	62	NuclAT_1	-	-
AM456_RS00355	44146..44526	-	381	Protein_57	DUF2726 domain-containing protein	-
AM456_RS00360	44720..44932	-	213	WP_104888872.1	hypothetical protein	-
AM456_RS00365	45068..45628	-	561	WP_104888873.1	fertility inhibition protein FinO	-
AM456_RS00370	45683..46429	-	747	WP_104888874.1	conjugal transfer pilus acetylase TraX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	faeI / faeH / faeF / faeE / faeD / faeC	1..90045	90045

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (30-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (15-99)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17659.41 Da
• Isoelectric Point: 7.1036

>T95638 WP_000405244.1 NZ_CP026754:c43103-42621 [Escherichia coli]
MEINVTAPALLTDEHILQPFDCGNEVLSNWLHGRAMKNQMLNASRTFVICLEGTLRVVGYYSLATGSVTHAELGRSLRHN
MPNPVPVVLLGRLAVDVCTQGHGFGKWLLSDAIHRVVNLADQVGIKAVMVHAIDDDARAFYERFGFVQSVVAPNTLFYKV

Nucleotide

• Length: 483 bp

>T95638 NZ_CP026754:c43103-42621 [Escherichia coli]
ATGGAAATAAATGTCACCGCACCGGCATTACTGACGGATGAGCATATACTTCAGCCATTTGATTGTGGGAATGAAGTGTT
AAGTAACTGGTTACATGGTCGCGCTATGAAAAACCAGATGCTCAATGCTTCCCGCACGTTCGTCATTTGCCTGGAGGGTA
CTTTACGTGTTGTGGGGTACTACTCATTAGCTACCGGTTCCGTCACTCATGCCGAACTTGGGCGCAGTTTGCGGCATAAC
ATGCCCAATCCTGTTCCTGTTGTTTTGTTAGGGCGTCTGGCTGTTGATGTTTGTACTCAGGGGCATGGTTTTGGTAAATG
GCTGCTGAGTGATGCCATTCATCGGGTTGTCAATCTGGCTGATCAGGTTGGTATTAAGGCTGTTATGGTTCATGCAATTG
ATGATGATGCCAGAGCGTTTTATGAGCGTTTTGGTTTTGTCCAGTCAGTTGTGGCACCAAATACCTTGTTTTATAAAGTC
TGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11602.19 Da
• Isoelectric Point: 4.4569

>AT95638 WP_001346256.1 NZ_CP026754:c43396-43094 [Escherichia coli]
MEVVMPTTASIRKTPRENQINIRATDEERAVIDYAASLVNKNRTDFIIELAYQEAKNIILDQRLFVLDDECYDSFITQLE
APVQNAEGRERLMAVKPEWK

Nucleotide

• Length: 303 bp

>AT95638 NZ_CP026754:c43396-43094 [Escherichia coli]
ATGGAGGTTGTTATGCCTACTACTGCAAGCATCCGTAAAACACCGCGAGAAAATCAGATTAACATCCGGGCAACGGATGA
GGAACGAGCTGTTATTGATTATGCCGCTAGCCTGGTCAATAAGAACCGTACTGATTTCATCATTGAACTGGCTTACCAGG
AAGCTAAAAATATCATCCTGGATCAACGTTTATTTGTGCTGGATGATGAATGTTATGATTCATTTATTACGCAACTTGAA
GCTCCTGTGCAGAATGCTGAAGGGCGTGAACGTCTGATGGCGGTAAAGCCTGAATGGAAATAA