Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 38714..39357 Replicon plasmid tig00000084_pilon
Accession NZ_CP026753
Organism Klebsiella pneumoniae strain AR_0066

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag AM445_RS28750 Protein ID WP_001044770.1
Coordinates 38941..39357 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag AM445_RS28745 Protein ID WP_001261282.1
Coordinates 38714..38944 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM445_RS28715 34286..35152 - 867 WP_004118283.1 replication initiation protein -
AM445_RS29695 35687..35791 + 105 WP_032409716.1 hypothetical protein -
AM445_RS28720 35920..36177 + 258 WP_000764642.1 hypothetical protein -
AM445_RS28725 36235..37011 - 777 WP_000015958.1 site-specific integrase -
AM445_RS28730 37008..37751 - 744 WP_000129823.1 hypothetical protein -
AM445_RS28735 37802..38152 - 351 WP_000493378.1 hypothetical protein -
AM445_RS29700 38296..38757 - 462 WP_072202616.1 hypothetical protein -
AM445_RS28745 38714..38944 + 231 WP_001261282.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
AM445_RS28750 38941..39357 + 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
AM445_RS28755 39431..40141 + 711 WP_004152334.1 ATP-binding protein -
AM445_RS28760 40175..40872 + 698 Protein_53 IS1 family transposase -
AM445_RS28765 40957..41817 - 861 WP_000027057.1 class A broad-spectrum beta-lactamase TEM-1 -
AM445_RS28770 42000..42317 - 318 Protein_55 recombinase family protein -
AM445_RS28775 42517..43341 - 825 WP_000722315.1 oxacillin-hydrolyzing class D beta-lactamase OXA-9 -
AM445_RS28780 43401..44189 - 789 WP_001206315.1 ANT(3'')-Ia family aminoglycoside nucleotidyltransferase AadA1 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid ARR-3 / ere(A) / ant(3'')-Ia / cmlA1 / qacE / sul1 / aph(6)-Id / aph(3'')-Ib / sul2 / blaTEM-1A / blaOXA-9 / aac(6')-Ib / blaCTX-M-15 / aac(3)-IId / blaSHV-187 - 1..70730 70730
- inside Integron - - 3..70635 70632


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T95636 WP_001044770.1 NZ_CP026753:38941-39357 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T95636 NZ_CP026753:38941-39357 [Klebsiella pneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT95636 WP_001261282.1 NZ_CP026753:38714-38944 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT95636 NZ_CP026753:38714-38944 [Klebsiella pneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References