Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 48202..48938 Replicon plasmid tig00000080_pilon
Accession NZ_CP026752
Organism Klebsiella pneumoniae strain AR_0066

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag AM445_RS27525 Protein ID WP_003026803.1
Coordinates 48202..48684 (-) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag AM445_RS27530 Protein ID WP_003026799.1
Coordinates 48672..48938 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM445_RS27510 43839..44801 + 963 WP_004152113.1 M48 family metalloprotease -
AM445_RS27515 44889..46143 + 1255 Protein_47 IS3 family transposase -
AM445_RS27520 46644..47990 - 1347 WP_020314316.1 ISNCY family transposase -
AM445_RS27525 48202..48684 - 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
AM445_RS27530 48672..48938 - 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
AM445_RS27535 49114..49368 - 255 WP_004152108.1 hypothetical protein -
AM445_RS27540 49444..49701 - 258 WP_004152107.1 hypothetical protein -
AM445_RS27545 49750..49953 - 204 WP_004152106.1 hemolysin expression modulator Hha -
AM445_RS27550 49987..50355 - 369 WP_004152105.1 hypothetical protein -
AM445_RS27555 50399..50893 - 495 WP_004152104.1 DNA-binding protein -
AM445_RS27560 50924..51499 - 576 WP_004152103.1 hypothetical protein -
AM445_RS27565 51487..51756 - 270 WP_004152102.1 hypothetical protein -
AM445_RS27570 52114..52464 + 351 WP_004152101.1 As(III)-sensing metalloregulatory transcriptional repressor ArsR -
AM445_RS27575 52514..52876 + 363 WP_004152100.1 arsenite efflux transporter metallochaperone ArsD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid catA1 - 1..211813 211813


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T95635 WP_003026803.1 NZ_CP026752:c48684-48202 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T95635 NZ_CP026752:c48684-48202 [Klebsiella pneumoniae]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT95635 WP_003026799.1 NZ_CP026752:c48938-48672 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT95635 NZ_CP026752:c48938-48672 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References