Detailed information of TA system TA095614

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 35667..36195
• Replicon: plasmid pOXA58_005133
• Accession: NZ_CP026749
• Organism: Acinetobacter baumannii strain WCHAB005133

Toxin (Protein)

• Gene name: relE
• Uniprot ID: N8SCV7
• Locus tag: C5B74_RS00250
• Protein ID: WP_004733298.1
• Coordinates: 35902..36195 (+)
• Length: 98 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A1E8DZ10
• Locus tag: C5B74_RS00245
• Protein ID: WP_026438268.1
• Coordinates: 35667..35912 (+)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C5B74_RS00220	30802..31632	-	831	Protein_33	IS91-like element ISVsa3 family transposase	-
C5B74_RS00225	31696..32400	+	705	WP_001067784.1	IS6-like element IS1006 family transposase	-
C5B74_RS00230	32454..32708	+	255	Protein_35	recombinase family protein	-
C5B74_RS00235	32954..33805	-	852	WP_002115246.1	universal stress protein	-
C5B74_RS00240	33818..35305	-	1488	WP_005146104.1	SulP family inorganic anion transporter	-
C5B74_RS00245	35667..35912	+	246	WP_026438268.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
C5B74_RS00250	35902..36195	+	294	WP_004733298.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C5B74_RS00255	36261..36575	-	315	WP_005108843.1	hypothetical protein	-
C5B74_RS00260	36682..37113	+	432	WP_104915069.1	hypothetical protein	-
C5B74_RS00265	37103..37429	+	327	WP_019838418.1	hypothetical protein	-
C5B74_RS00275	37480..38699	+	1220	WP_104915068.1	IS3 family transposase	-
C5B74_RS00280	38940..39236	+	297	WP_004647749.1	type II toxin-antitoxin system RelE/ParE family toxin	-
C5B74_RS00285	39238..39564	+	327	WP_104915067.1	putative addiction module antidote protein	-
C5B74_RS00290	39617..39820	-	204	WP_004896916.1	hypothetical protein	-
C5B74_RS00300	40200..40511	+	312	WP_104915071.1	BrnT family toxin	-
C5B74_RS00305	40474..40788	+	315	WP_019838467.1	BrnA antitoxin family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	blaOXA-58 / floR / sul2	-	1..42455	42455
	-	inside	IScluster/Tn	-	-	37480..42454	4974

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (8-84)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-74)

Sequences

Toxin

Protein

• Length: 98 a.a.
• Molecular weight: 11252.46 Da
• Isoelectric Point: 10.4456

>T95614 WP_004733298.1 NZ_CP026749:35902-36195 [Acinetobacter baumannii]
MTYKLLRHKDFTAEWEKLPSAIRDQFKKKLAKVIEQPHIPKNMLKGDLAGCYKIKLLKAGVRLVYQVKDDQVVILLITVG
KRADSIVYDEAKKRIKD

Nucleotide

• Length: 294 bp

>T95614 NZ_CP026749:35902-36195 [Acinetobacter baumannii]
ATGACTTATAAGCTTTTACGTCATAAGGACTTCACTGCAGAATGGGAAAAGCTTCCTAGTGCTATACGAGATCAATTCAA
GAAAAAATTAGCTAAGGTAATAGAGCAACCACATATTCCAAAGAATATGCTTAAAGGTGATCTTGCAGGCTGCTATAAAA
TAAAATTATTAAAAGCTGGTGTCCGACTTGTTTACCAGGTCAAAGATGATCAGGTAGTTATTTTGCTAATTACCGTTGGA
AAACGAGCTGATAGCATTGTTTATGATGAAGCAAAAAAGCGGATAAAAGACTAA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 9248.61 Da
• Isoelectric Point: 4.2821

>AT95614 WP_026438268.1 NZ_CP026749:35667-35912 [Acinetobacter baumannii]
MATVNFRVDEALKEKSYSILKEQGIAPTEFFTNILEYIATTGKLPVQKALLSEEDTELLAIVRKRVNDPKEMFEEVTLDD
L

Nucleotide

• Length: 246 bp

>AT95614 NZ_CP026749:35667-35912 [Acinetobacter baumannii]
ATGGCTACTGTTAATTTCAGGGTCGATGAAGCCCTTAAAGAAAAGTCTTATTCCATCCTAAAAGAACAAGGAATTGCACC
TACTGAGTTCTTTACTAATATTCTTGAATATATTGCTACGACTGGAAAGCTTCCAGTACAAAAAGCTTTACTCTCAGAAG
AGGATACCGAGCTATTAGCGATTGTTCGTAAACGTGTTAATGATCCTAAAGAGATGTTCGAGGAAGTCACATTAGATGAC
TTATAA

Structures

Toxin

• AlphaFold DB: N8SCV7

Antitoxin

• AlphaFold DB: A0A1E8DZ10