Detailed information of TA system TA095612

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC(toxin)
• Location: 759808..760481
• Replicon: chromosome
• Accession: NZ_CP026746
• Organism: Nocardia cyriacigeorgica strain MDA3349 isolate MDA3349 ancestor

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: C5B73_RS03975
• Protein ID: WP_036533092.1
• Coordinates: 759808..760227 (-)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: C5B73_RS03980
• Protein ID: WP_036533093.1
• Coordinates: 760227..760481 (-)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C5B73_RS03950	754897..755772	-	876	WP_104361435.1	M23 family metallopeptidase	-
C5B73_RS03955	756004..757050	+	1047	WP_174343892.1	nitronate monooxygenase	-
C5B73_RS03960	757108..757500	+	393	WP_036533089.1	hypothetical protein	-
C5B73_RS03965	757649..759295	-	1647	WP_036533091.1	long-chain fatty acid--CoA ligase	-
C5B73_RS03975	759808..760227	-	420	WP_036533092.1	type II toxin-antitoxin system VapC family toxin	Toxin
C5B73_RS03980	760227..760481	-	255	WP_036533093.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
C5B73_RS03985	760860..762002	+	1143	WP_104361436.1	acyl-CoA dehydrogenase family protein	-
C5B73_RS03990	762079..763545	+	1467	WP_104361437.1	NAD(P)/FAD-dependent oxidoreductase	-
C5B73_RS03995	763549..763770	-	222	WP_156090791.1	hypothetical protein	-
C5B73_RS04005	763981..765195	-	1215	WP_197538404.1	site-specific integrase	-
C5B73_RS04010	765192..765389	-	198	WP_048832752.1	AlpA family phage regulatory protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-124)

Antitoxin

• Antitoxin VapB-like (1-83)

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 15224.18 Da
• Isoelectric Point: 4.4859

>T95612 WP_036533092.1 NZ_CP026746:c760227-759808 [Nocardia cyriacigeorgica]
MVIDTSAVVAMLNDEPEAPRYEAAIEADPTRLMSAATYLETSIVIESRFGEAGGRELDLWLHRAGVDLVAVHPEHAHIAR
GAYRQYGKGRHPAALNYGDCFSYALSKVSGEPLLFKGDDFPRTDIMAVPVPEVEEVVEP

Nucleotide

• Length: 420 bp

>T95612 NZ_CP026746:c760227-759808 [Nocardia cyriacigeorgica]
ATGGTCATAGATACTTCAGCTGTCGTCGCCATGTTGAATGACGAACCTGAAGCCCCGCGGTATGAGGCTGCGATCGAGGC
GGATCCAACGCGCCTGATGTCGGCGGCGACATACCTCGAGACGTCGATCGTCATCGAGAGTCGATTCGGCGAAGCCGGAG
GGCGTGAGCTCGATCTGTGGCTACATCGTGCGGGTGTGGATCTCGTGGCCGTGCATCCCGAGCACGCGCACATCGCGCGT
GGTGCATATCGCCAGTACGGAAAGGGGCGTCATCCGGCGGCGCTGAACTACGGTGACTGCTTCTCGTACGCGCTGTCCAA
GGTGAGCGGGGAGCCGCTCCTGTTCAAGGGCGATGACTTTCCGCGCACCGACATCATGGCTGTGCCGGTGCCGGAGGTGG
AAGAGGTCGTCGAGCCGTAG

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9156.50 Da
• Isoelectric Point: 5.8625

>AT95612 WP_036533093.1 NZ_CP026746:c760481-760227 [Nocardia cyriacigeorgica]
MALSIKHPEADRLARELAAQTGETLTEAVVIALRERLARKAGQARAVPVREELAAIRRRCAALPVVDDRTPDEILGYDEN
GLPA

Nucleotide

• Length: 255 bp

>AT95612 NZ_CP026746:c760481-760227 [Nocardia cyriacigeorgica]
ATGGCATTGAGTATCAAGCATCCAGAGGCCGATCGTCTGGCGCGTGAACTCGCGGCTCAGACGGGGGAGACGCTGACCGA
GGCGGTTGTCATCGCGTTGCGTGAGCGCTTGGCGCGTAAGGCAGGACAGGCCAGAGCAGTGCCGGTGCGCGAGGAGCTCG
CCGCGATTCGACGCCGGTGCGCGGCACTTCCGGTGGTCGACGATCGCACCCCCGATGAGATTCTCGGCTACGACGAGAAT
GGGCTGCCTGCCTGA