Detailed information of TA system TA095611

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazF-pemI/MazF(toxin)
• Location: 1768253..1768889
• Replicon: chromosome
• Accession: NZ_CP026736
• Organism: Priestia megaterium strain YC4-R4

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: D5DWT4
• Locus tag: C2I28_RS09230
• Protein ID: WP_013055004.1
• Coordinates: 1768539..1768889 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: D5DWT3
• Locus tag: C2I28_RS09225
• Protein ID: WP_013055003.1
• Coordinates: 1768253..1768534 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C2I28_RS09200	1763619..1764590	+	972	WP_033580801.1	UV DNA damage repair endonuclease UvsE	-
C2I28_RS09205	1764599..1765201	-	603	WP_028411882.1	rhomboid family intramembrane serine protease	-
C2I28_RS09210	1765266..1765631	+	366	WP_028411881.1	holo-ACP synthase	-
C2I28_RS09215	1765690..1766748	+	1059	WP_033580802.1	outer membrane lipoprotein carrier protein LolA	-
C2I28_RS09220	1766862..1768052	+	1191	WP_034327253.1	alanine racemase	-
C2I28_RS09225	1768253..1768534	+	282	WP_013055003.1	hypothetical protein	Antitoxin
C2I28_RS09230	1768539..1768889	+	351	WP_013055004.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
C2I28_RS09235	1769047..1769883	+	837	WP_013055005.1	STAS domain-containing protein	-
C2I28_RS09240	1769886..1770242	+	357	WP_013055006.1	STAS domain-containing protein	-
C2I28_RS09245	1770246..1770647	+	402	WP_013055007.1	anti-sigma regulatory factor	-
C2I28_RS09250	1770661..1771671	+	1011	WP_013055008.1	PP2C family protein-serine/threonine phosphatase	-
C2I28_RS09255	1771731..1772063	+	333	WP_013055009.1	anti-sigma factor antagonist	-
C2I28_RS09260	1772060..1772545	+	486	WP_025753516.1	anti-sigma B factor RsbW	-
C2I28_RS09265	1772511..1773305	+	795	WP_028411878.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12991.96 Da
• Isoelectric Point: 4.8781

>T95611 WP_013055004.1 NZ_CP026736:1768539-1768889 [Priestia megaterium]
MVVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDRVDEALQISVGLIDF

Nucleotide

• Length: 351 bp

>T95611 NZ_CP026736:1768539-1768889 [Priestia megaterium]
TTGGTAGTCAAACGTGGCGACGTTTATTTTGCTGACCTATCTCCTGTTGTTGGCTCAGAACAAGGAGGCGTTCGCCCTGT
ACTTGTCATTCAAAATGATATAGGCAATCGTTTTAGCCCAACTGTTATTGTAGCTGCGATCACTGCTCAAATTCAAAAGG
CAAAGTTACCTACGCATGTTGAAATTGATGCAAAGCGCTATGGATTTGAGCGAGATTCAGTTATTTTACTAGAACAAATT
CGTACTATTGATAAACAGAGGCTAACAGACAAGATTACCCATTTAGATGATGAAATGATGGACAGAGTGGATGAAGCCTT
GCAAATCAGTGTAGGACTTATCGACTTTTAG

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10691.22 Da
• Isoelectric Point: 8.5339

>AT95611 WP_013055003.1 NZ_CP026736:1768253-1768534 [Priestia megaterium]
VSEASATTEILVRLPKNLVSELDLMVKQENGNRSDFIHQATKLYLRERKKRHIRESMRRGYMEMAKINLNIASEAFLAEY
EADHTVDRLVSGG

Nucleotide

• Length: 282 bp

>AT95611 NZ_CP026736:1768253-1768534 [Priestia megaterium]
GTGTCTGAAGCGAGCGCAACTACAGAAATCCTAGTAAGATTACCGAAAAATTTAGTATCGGAGCTCGATCTTATGGTTAA
ACAAGAAAATGGCAACCGAAGTGATTTTATTCACCAAGCAACAAAATTGTACCTTCGTGAGCGAAAAAAACGCCATATTC
GCGAATCAATGAGACGTGGATATATGGAGATGGCAAAGATAAACTTAAATATTGCTTCCGAGGCTTTTCTAGCAGAGTAT
GAAGCTGATCATACTGTGGATCGTTTAGTAAGCGGGGGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A0B6AMB9