Detailed information of TA system TA095609

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC(toxin)
• Location: 158686..159299
• Replicon: chromosome
• Accession: NZ_CP026734
• Organism: Brevibacterium linens strain ATCC 19391

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: CXR29_RS00720
• Protein ID: WP_127365211.1
• Coordinates: 158892..159299 (+)
• Length: 136 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A2H1KFP4
• Locus tag: CXR29_RS00715
• Protein ID: WP_025777403.1
• Coordinates: 158686..158895 (+)
• Length: 70 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CXR29_RS00695	154499..156535	+	2037	WP_127363574.1	phenylacetic acid degradation bifunctional protein PaaZ	-
CXR29_RS00700	156680..157102	-	423	WP_127363575.1	hypothetical protein	-
CXR29_RS00705	157042..157734	-	693	WP_206516241.1	acylglycerol kinase family protein	-
CXR29_RS00710	157641..158711	-	1071	WP_127363577.1	hypothetical protein	-
CXR29_RS00715	158686..158895	+	210	WP_025777403.1	hypothetical protein	Antitoxin
CXR29_RS00720	158892..159299	+	408	WP_127365211.1	PIN domain nuclease	Toxin
CXR29_RS00725	159321..160049	-	729	WP_127363578.1	TetR/AcrR family transcriptional regulator	-
CXR29_RS00730	160136..161431	-	1296	WP_127363579.1	AMP-binding protein	-
CXR29_RS00735	161591..162118	-	528	WP_127363580.1	hydroxyphenylacetyl-CoA thioesterase PaaI	-
CXR29_RS00740	162670..163689	+	1020	WP_127363581.1	1,2-phenylacetyl-CoA epoxidase subunit A	-
CXR29_RS00745	163686..164027	+	342	WP_025777409.1	1,2-phenylacetyl-CoA epoxidase subunit B	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-124)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 136 a.a.
• Molecular weight: 14711.84 Da
• Isoelectric Point: 4.5117

>T95609 WP_127365211.1 NZ_CP026734:158892-159299 [Brevibacterium linens]
VKGLSWLIDKSALVRVGRSGEAEQWLDRINRGLVQISIPTLLEVGYSATSPTDWSAAIETPPISLMPLAGITPAVERRAI
EVQGILADKGHHRAPSVPDLLIAAIGEVNGLVVLHQDKDFELIAEITEQEFERID

Nucleotide

• Length: 408 bp

>T95609 NZ_CP026734:158892-159299 [Brevibacterium linens]
GTGAAGGGGCTCTCGTGGCTCATTGACAAATCTGCGTTGGTTCGAGTCGGACGCAGCGGTGAGGCCGAGCAGTGGCTGGA
CCGGATCAACCGTGGTCTGGTCCAGATCTCAATCCCGACTCTGCTGGAAGTAGGGTATTCGGCGACGAGCCCAACAGACT
GGTCCGCAGCCATCGAAACACCCCCGATTTCCCTGATGCCACTTGCAGGGATTACACCGGCGGTCGAGAGAAGAGCCATT
GAGGTGCAGGGCATCCTGGCTGACAAGGGTCATCACCGCGCGCCGTCGGTCCCAGATCTGCTTATTGCCGCGATCGGCGA
GGTGAATGGGCTTGTTGTCCTGCATCAGGACAAGGATTTCGAGTTGATCGCTGAAATCACTGAGCAGGAGTTCGAGCGGA
TCGACTGA

Antitoxin

Protein

• Length: 70 a.a.
• Molecular weight: 7922.87 Da
• Isoelectric Point: 5.1037

>AT95609 WP_025777403.1 NZ_CP026734:158686-158895 [Brevibacterium linens]
MTDVLIRNISDETLSRVDAMARRAGLSRAEYLRRTIDREALRSDEAATIEDLRKFDRLADEQHMSGAWG

Nucleotide

• Length: 210 bp

>AT95609 NZ_CP026734:158686-158895 [Brevibacterium linens]
ATGACTGATGTGCTGATCCGAAACATTTCGGATGAGACTCTGTCGAGAGTCGATGCGATGGCTCGGCGCGCGGGGTTGTC
TCGTGCTGAATATCTGCGGCGGACGATTGATCGGGAGGCGCTTCGCTCCGATGAAGCGGCGACTATCGAGGACCTGCGCA
AATTCGATCGACTGGCGGATGAGCAGCATATGAGTGGGGCGTGGGGGTGA

Structures

Antitoxin

• AlphaFold DB: A0A2H1KFP4