Detailed information of TA system TA095603

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK-RelB
• Location: 812983..813538
• Replicon: chromosome
• Accession: NZ_CP026729
• Organism: Bifidobacterium catenulatum subsp. kashiwanohense strain APCKJ1

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: S2ZXD7
• Locus tag: BKKJ1_RS03460
• Protein ID: WP_003829109.1
• Coordinates: 812983..813306 (-)
• Length: 108 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: BKKJ1_RS03465
• Protein ID: WP_154303659.1
• Coordinates: 813293..813538 (-)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BKKJ1_RS03435	808464..808762	-	299	Protein_651	IS256 family transposase	-
BKKJ1_RS03440	809233..810498	-	1266	WP_154303657.1	Fic family protein	-
BKKJ1_RS03445	811042..811407	-	366	WP_065474609.1	YccF domain-containing protein	-
BKKJ1_RS03450	811645..812031	+	387	Protein_654	class I SAM-dependent methyltransferase	-
BKKJ1_RS03455	812152..812766	+	615	WP_154303658.1	cupin domain-containing protein	-
BKKJ1_RS03460	812983..813306	-	324	WP_003829109.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
BKKJ1_RS03465	813293..813538	-	246	WP_154303659.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
BKKJ1_RS03470	813712..814968	+	1257	WP_003829116.1	HipA domain-containing protein	-
BKKJ1_RS03475	815233..815424	+	192	WP_154304378.1	XRE family transcriptional regulator	-
BKKJ1_RS03480	815608..816867	-	1260	WP_154303660.1	MFS transporter	-
BKKJ1_RS03485	817024..817686	-	663	WP_154304379.1	TetR/AcrR family transcriptional regulator	-
BKKJ1_RS10695	818148..818333	+	186	WP_033501981.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	803206..818333	15127

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (2-103)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (5-56)

Sequences

Toxin

Protein

• Length: 108 a.a.
• Molecular weight: 11859.67 Da
• Isoelectric Point: 5.0771

>T95603 WP_003829109.1 NZ_CP026729:c813306-812983 [Bifidobacterium catenulatum subsp. kashiwanohense]
MKRGEIWTLQADGYASKPRPVVIVQSDAVDRFDSVITCLLTSYDSSDIDTRVRLEPSPENGLNKVSYVMTDKIVTVDRKL
LGYQVGVVDDAAMANIGRQLMRVLGLL

Nucleotide

• Length: 324 bp

>T95603 NZ_CP026729:c813306-812983 [Bifidobacterium catenulatum subsp. kashiwanohense]
ATGAAACGAGGTGAGATTTGGACGTTGCAGGCCGATGGCTACGCCAGCAAGCCACGGCCGGTTGTCATCGTGCAGAGCGA
TGCCGTCGATCGGTTCGATTCCGTCATCACTTGTCTGCTGACCTCATACGATTCCTCCGACATCGACACGCGGGTCCGAT
TGGAACCAAGTCCGGAGAACGGCCTGAACAAAGTGAGTTACGTGATGACGGACAAGATCGTCACCGTCGACCGCAAACTG
CTGGGATACCAAGTCGGCGTGGTTGATGATGCAGCCATGGCGAATATCGGCCGTCAGCTCATGCGGGTTCTAGGTTTGCT
GTGA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 9306.39 Da
• Isoelectric Point: 4.7719

>AT95603 WP_154303659.1 NZ_CP026729:c813538-813293 [Bifidobacterium catenulatum subsp. kashiwanohense]
MSVQIATRVNDEQAALFKETTRQLGTTPADALRMFISAFNDYRGFPYEVRLPRNDVEPFASERDATEYASRLALRMSDET
R

Nucleotide

• Length: 246 bp

>AT95603 NZ_CP026729:c813538-813293 [Bifidobacterium catenulatum subsp. kashiwanohense]
ATGAGCGTACAGATTGCCACGCGAGTTAATGACGAGCAGGCTGCGTTGTTTAAGGAGACGACCAGGCAGCTGGGTACCAC
CCCGGCCGATGCCCTGCGTATGTTCATCAGCGCGTTCAATGATTACCGTGGGTTCCCCTATGAGGTGCGTTTGCCACGAA
ATGACGTGGAGCCGTTTGCGTCCGAGCGGGACGCTACTGAGTATGCCAGTCGTCTCGCCTTGAGGATGTCCGATGAAACG
AGGTGA

Structures

Toxin

• AlphaFold DB: A0A087B6Q7