Detailed information of TA system TA095598

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-Phd
• Location: 64517..65118
• Replicon: plasmid p266917_2_02
• Accession: NZ_CP026725
• Organism: Escherichia coli strain 266917_2

Toxin (Protein)

• Gene name: doc
• Uniprot ID: V0AJ64
• Locus tag: C4R22_RS29900
• Protein ID: WP_001216034.1
• Coordinates: 64517..64897 (-)
• Length: 127 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: U9YQH9
• Locus tag: C4R22_RS29905
• Protein ID: WP_001190712.1
• Coordinates: 64897..65118 (-)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C4R22_RS29875	59957..61441	-	1485	WP_000124155.1	hypothetical protein	-
C4R22_RS29880	61441..62634	-	1194	WP_104858488.1	terminase	-
C4R22_RS29885	62721..63173	-	453	WP_001312282.1	late promoter-activating protein (Gp10)	-
C4R22_RS29890	63262..64305	-	1044	WP_052870176.1	DUF968 domain-containing protein	-
C4R22_RS29895	64333..64512	-	180	WP_001339207.1	hypothetical protein	-
C4R22_RS29900	64517..64897	-	381	WP_001216034.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
C4R22_RS29905	64897..65118	-	222	WP_001190712.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
C4R22_RS29910	65191..65580	-	390	WP_104858489.1	DNA repair protein	-
C4R22_RS29915	65755..66327	+	573	WP_001133670.1	hypothetical protein	-
C4R22_RS29920	66334..66585	-	252	WP_001667237.1	DNA polymerase III subunit theta	-
C4R22_RS29930	67247..67609	-	363	WP_097494657.1	hypothetical protein	-
C4R22_RS29935	67606..68538	-	933	WP_097494658.1	hypothetical protein	-
C4R22_RS29940	68520..68894	-	375	WP_074505450.1	hypothetical protein	-
C4R22_RS29945	68901..69194	-	294	WP_000269001.1	hypothetical protein	-
C4R22_RS29950	69373..69606	-	234	WP_000517420.1	hypothetical protein	-
C4R22_RS29955	69683..69943	-	261	WP_000969524.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..97124	97124

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (2-123)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-51)

Sequences

Toxin

Protein

• Length: 127 a.a.
• Molecular weight: 13615.32 Da
• Isoelectric Point: 5.1514

>T95598 WP_001216034.1 NZ_CP026725:c64897-64517 [Escherichia coli]
MRHISPEELIALHDANINRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Nucleotide

• Length: 381 bp

>T95598 NZ_CP026725:c64897-64517 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAACCGCTACGGCGGCCTGCCGGGAATGTC
AGATCCGGGTAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTGGTGGCTACAGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGCGCG
CTGCTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACTGTAGGCGCTGCGACTGG
CGAGATATCTGTATCTTCTGTCGCCGACACGTTACGTAGATTGTATGGTTCTGCGGAGTAG

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8133.10 Da
• Isoelectric Point: 4.7708

>AT95598 WP_001190712.1 NZ_CP026725:c65118-64897 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Nucleotide

• Length: 222 bp

>AT95598 NZ_CP026725:c65118-64897 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCGCGCGGCAACCTTTCTGAAGTGCTCAACAATGTTGAAGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Structures

Toxin

• AlphaFold DB: V0AJ64

Antitoxin

• AlphaFold DB: A0A829CJB6